结直肠癌转移相关microRNA探讨

Metastasis related microRNA in colorectal cancer

目的通过对结直肠癌患者血清进行实时定量PCR检测,筛选出结直肠癌转移相关的miRNA。方法通过文献检索,筛选出11个结直肠癌转移相关的miRNA(miRs-31,335,206,141,126,200b,200c,21,Let7a,Let 7b和Let 7c),收集2007年7月至2013年4月北京友谊医院收治的术前留置血清的结直肠癌转移(IV期)病例共计108例,其中结直肠肝转移患者72例,其他脏器转移患者36例;并纳入2008年1月至2010年6月术前留置血清的局灶性结直肠癌(L-CRC)(I-III期)病例共计116例作为对照组进行研究。通过对两组患者血清样本进行实时定量PCR检测结果找出结直肠癌转移相关miRNA。结果筛选出11个转移相关的miRNA中的7个miRNA(miRs-31、141、126、21、Let7a、Let7b和Let7c)可以从血清中被检测到。与局灶性结直肠癌患者相比,miR-141,miR-126,Let-7a和miR-21在转移性结直肠癌患者的血清中表达具有显著性差异(P<0.0001,P<0.0001,P=0.0120和P<0.0001),可以作为结直肠癌转移相关miRNA。结论 7个转移相关MiRNAs:miRs-31、141、126、21、Let7a、Let7b和Let7c可以从结直肠癌患者血清中被检测到;miR-141,miR-126,Let-7a和miR-21可以作为结直肠癌转移相关miRNA。

Objective To investigate the distribution of the difference in the expression of miRNAs in patients with colorectal liver metastases. Methods We reviewed the literature in search for studies reporting on miRNA expression profiles of motile or invasive cells and obtained 11 miRNA which is related with metastasis in colorectal cancer（ miRs- 31,335,206,141,126,200 b,200c,21,Let7 a,Let 7b and Let 7c）. Serum samples（ n = 224） from CRC patients that had been surgically treated were obtained from Beijing Friendship Hospital,including 116 consecutive patients of localized CRC（ L- CRC）（ stage I- III） between January 2008 and June 2010,and 108 consecutive patients of metastatic CRC between September 2007 and April 2013. Results Seven（ miRs- 31,141,126,21,Let7 a,Let7b and Let7c） of 11 tested miRNAs could be detected from serum. Four miRNAs（ miR- 126,miR- 141,Let- 7a and miR- 21） were detected as metastasis- associated miRNAs（ P 〈 0.0001,P 〈 0. 0001,P = 0. 0120 and P 〈 0. 0001）. Conclusion These selected candidate miRNAs by q PCR and detected the expressions of miRs- 31,141,126,21,Let7 a,Let7b and Let7 c in serum samples. miR- 141,miR- 126,Let- 7a and miR- 21 were associated with metastasis of colorectal.