空间爬行运动训练对脑缺血幼鼠心理行为的影响

Psychological behavior effects of space creep exercise training for early age cerebral ischemia rats

目的：观察空间爬行运动训练对脑缺血幼鼠心理行为的影响。方法59只7～8日龄新生鼠随机分为对照组20只，仅做颈部皮肤的切开缝合，未结扎双侧颈总动脉；脑缺血组20只，结扎双侧颈总动脉；脑缺血运动组19只，结扎双侧颈总动脉后4 d，给本组幼鼠在自制的空间运动架上进行早期空间运动训练4周。对照组和脑缺血组不给与空间爬行训练。应用动物行为学平台（转棒仪和旷场试验）对38～40日龄幼鼠进行运动和行为功能测定。结果（1）脑缺血运动组和对照组幼鼠降落的潜伏期明显长于脑缺血组（t＝4．159，P＜0．01；t＝4．696，P＜0．01），而对照组和脑缺血运动组的幼鼠运动降落的潜伏期长短差异无统计学意义（t＝1．418）；（2）在旷场实验中3组幼鼠运动的总路程，以及平均速度比较差异均无统计学意义；（3）脑缺血运动组和对照组幼鼠的跨格次数、周边活动时间和距离明显长于脑缺血组（ t＝4．848，P＜0．001，t＝6．583，P＜0．001；t＝2．764，P＜0．05，t＝3．988，P＜0．01；t＝3．102，P＜0．05，t＝3．258，P＜0．05），而对照组和脑缺血运动组的幼鼠的跨格次数、周边活动时间和距离长短差异无统计学意义；（4）脑缺血运动组和对照组幼鼠中心活动时间及路程明显短于脑缺血组（t＝4．881，P＜0．01；t＝3．533，P＜0．01；t＝3．783，P＜0．01，t＝3．142，P＜0．05），脑缺血运动组和对照组幼鼠中心活动的时间和距离比较差异无统计学意义。结论脑缺血幼鼠表现情绪异常，运动行为状态明显受损，早期空间爬行运动训练可明显改善脑缺血幼鼠运动和协调能力及认知能力。

Objective To investigate the psychological behavior effects of space creep exercise training for early age cerebral ischemia rats.Methods 59 neonatal rats were randomly divided into 3 groups, 20 in control group with only the neck skin incision and suture, without bilateral common carotid artery ligation;20 in cerebral ischemia group with bilateral common carotid artery ligation;19 in cerebral ischemic exercise group , 4 days after bilateral common carotid artery ligation, given a 4 weeks creep space exercise training with homemade exercise rack.Control group and cerebral ischemia group did not receive exercise training.The Animal Behav-iour Science Platform （ Scotch instrument and open-field test） was used to measure the movement and behavior of these 38-40 days old rats.Results （1） The landing incubation period in cerebral ischemic exercise group and the control group was much longer than in cerebral ischemia group （t=4.159, P〈0.01;t=4.696, P〈0.01）, while the control group and cerebral ischemia exercise group had no significant difference （t=1.418）.（2） In the open field test, 3 groups had no significant difference in the total distance and average speed （all P＆gt;0.05）.（3） Peripheral activity time and distance in cerebral ischemic exercise group and the control group were much lon-ger than in cerebral ischemia group （t=4.848, P〈0.001, t=6.583, P〈0.001;t=2.764, P〈0.05, t=3.988, P〈0.01;t=3. 102, P〈0.05, t=3.258,P〈0.05） , while the number of cross-grid times, peripheral activity time and distance between the control group and cerebral ischemic exercise group had no significant difference.（4） Center activity time and distance in cerebral ischemic exer-cise group and the control were was much shorter than in cerebral ischemia group （t=4.881, P〈0.01;t=3.533, P〈0.01;t=3.783, P〈0.01, t =3.142, P 〈0.05）, while the cerebral ischemic exercise group and the control group had no dignificent difference. Conclusion For the cerebral ischemia rats, movement and behavior state are impaired, early space creeping exercise training can significantly improve the movement, coordination and cognitive abili-ties.