摘要
目的采用HPLC法测定不同产地新疆圆柏中的槲皮苷。方法色谱柱为Phenomenex ODS-A(250 mm×4.6 mm,5μm),流动相为乙腈-0.036 mol·L-1磷酸,梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长266 nm。结果槲皮苷2.2~88.0μg·m L-1与峰面积的线性关系良好(r=0.9999),加样回收率为101.38%,RSD=1.61%(n=6);样品溶液在32 h内稳定。结论所用方法操作简便、重复性好,可用于新疆圆柏药材的质量控制。
OBJECTIVE To detect quercitrin in Juniperus sabina L. from the different habitats by HPLC. METHODS The HPLC separation was performed on aPhenomenex ODS-A(250 mm×4.6 mm,5μm) in gradient elute mode with a mixture consisting of acetonitrile and 0. 036 mol. L- l phosphate acid solution at 30 ℃. The flow rate was 1.0 mL- min-1, and the detection wavelength was 266 nm. RESULTS The calibration curve of quercitrin was linear in the range of 2.2 - 88.0 μg· mL - 1 ( r = 0. 9999 ), and the average recovery of quercitrin was 101.38% with the RSD of 1.61% (n = 6). The properties of sample solution kept steady within 32 hours. CONCLUSION The method is simple and reproducible, and can be used for the quality control of J. sabina.
出处
《华西药学杂志》
CAS
CSCD
2015年第6期720-721,共2页
West China Journal of Pharmaceutical Sciences
基金
国家自然科学基金资助项目(批准号:81160515)