HPLC测定不同产地新疆圆柏中的槲皮苷

Determination of quercitrin in Juniperus sabina from the different habitats by HPLC

目的采用HPLC法测定不同产地新疆圆柏中的槲皮苷。方法色谱柱为Phenomenex ODS-A（250 mm×4.6 mm,5μm）,流动相为乙腈-0.036 mol·L-1磷酸,梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长266 nm。结果槲皮苷2.2～88.0μg·m L-1与峰面积的线性关系良好（r=0.9999）,加样回收率为101.38%,RSD=1.61%（n=6）;样品溶液在32 h内稳定。结论所用方法操作简便、重复性好,可用于新疆圆柏药材的质量控制。

OBJECTIVE To detect quercitrin in Juniperus sabina L. from the different habitats by HPLC. METHODS The HPLC separation was performed on aPhenomenex ODS-A（250 mm×4.6 mm,5μm） in gradient elute mode with a mixture consisting of acetonitrile and 0. 036 mol. L- l phosphate acid solution at 30 ℃. The flow rate was 1.0 mL- min-1, and the detection wavelength was 266 nm. RESULTS The calibration curve of quercitrin was linear in the range of 2.2 - 88.0 μg· mL - 1 （ r = 0. 9999 ）, and the average recovery of quercitrin was 101.38% with the RSD of 1.61% （n = 6）. The properties of sample solution kept steady within 32 hours. CONCLUSION The method is simple and reproducible, and can be used for the quality control of J. sabina.