D-serine合成相关丝氨酸消旋酶在大鼠和癫痫患者海马的分布与细胞定位

Distribution and cellular location of D-serine racemase(SR) in hippocampus of rats and patients with epilepsy

目的:观察D-serine合成相关的丝氨酸消旋酶(SR)在大鼠和癫痫病人海马组织的分布与细胞定位特点。方法:成年和幼龄SD大鼠灌流固定、冰冻切片癫痫患者海马硬化石蜡切片,进行SR、SR/NeuN、SR/GFAP免疫荧光染色、激光共聚焦显微镜观察和计数分析。结果:在成年和幼龄大鼠海马的CA1-3和齿状回内均分布有大量SR免疫阳性细胞分布,SR免疫阳性反应主要定位于神经元和星形胶质细胞;与成年相比,幼龄大鼠海马内SR免疫阳性的神经元数量相似,但SR/GFAP阳性星形胶质细胞的数量较多;SR阳性细胞在癫痫患者海马内也有大量分布,其数量明显多于脑出血患者。结论:SR定位于大鼠和癫痫患者海马神经元和星形胶质细胞,其数量分布与发育年龄和癫痫疾病状态呈现一定的相关性,提示D-serine的产生可能参与海马的发育和癫痫疾病过程。

Objective：To investigate the distribution and cellular location of D-serine racemase（SR） in hippocampus of rats and patients with temporal lobe epilepsy（TLE）.Methods：Under anesthesia the adult and young rats were perfused and sectioned in a frozen microtome,the hippocampus of TLE patients with hippocampal sclerosis（HS） were in paraffin section,then the section were processed to immunofluorescence histochemical staining and laser scanning confocal microscopy for SR,SR/NeuN,SR/GFAP.Results：The distribution of SR-immunoreactive cells were observed in both CA1-3 regions and dentate gyrus（DG） of hippocampus in young and adult rats,and SR-immunoreactivity was mainly localized in neurons and astrocytes.Compared with that of adults,the number of young rats exhibited similar pattern in SR/NeuNpositive neurons but more SR / GFAP-positive astrocytes in these regions.Compared with that of cerebral hemorrhage samples,patients with TLE-HS showed much more SR-positive neuronal cells in the hippocampus.Conclusion：SR is clearly expressed in the hippocampal neurons and astrocyte of rats and patients with TLE-HS,and its quantitative changes was related to aging and epilepsy states,suggesting that D-serine production might be involved in developing of hippocampus and pathogenesis of epilepsy.