摘要
目的了解2012—2014年江西省市售婴幼儿配方食品中蜡样芽胞杆菌的污染情况并对呕吐毒素基因进行分析。方法在全省市县中选取13个采样点,包括超市、百货商场、便利店、农贸市场、网店、批发市场,随机抽取婴幼儿配方食品397份,对蜡样芽胞杆菌进行检测和鉴定,同时应用叠氮溴化丙锭内参多重PCR方法检测分离株的呕吐毒素基因。结果 397份食品中蜡样芽胞杆菌阳性率为13.10%(52/397),其中2013年阳性率最高。不同采样点的阳性率差异有统计学意义(P<0.05),不同产地、不同流通环节和不同年龄段的阳性率差异无统计学意义(P>0.05),52份阳性食品中检出呕吐型蜡样芽胞杆菌2份,呕吐毒素阳性率为3.85%。结论江西省市售婴幼儿配方食品中存在蜡样芽胞杆菌及呕吐型蜡样芽胞杆菌的污染,存在一定的安全隐患,相关监管部门应继续加强监管,预防食源性疾病的发生。
Objective To investigate the contamination levels and emetic gene analysis of Bacillus cereus in infant formula from 2012 to 2014 in Jiangxi Province. Methods 397 food samples were collected randomly from supermarkets, department stores, convenience stores, the farmer＇s markets, online stores, wholesale markets in 13 selected cities and countries of the province. The emetic genes of isolated strains were analyzed by propidium monoazide multiplex PCR and internal amplification control. Results The detection rate was 13. 10% （52/397） , and the contamination was the highest in 2013. Different locations had significant difference （P 〈 0. 05） , while the different origins of the samples, circulation and infant formula for different ages had no statistical difference （P 〉 0. 05）. 2 emetic bacillus cereus were detected in 52 positive samples and the rate was 3.85%. Conclusion The contamination for Bacillus cereus and emetic Bacillus cereus in infant formula in retail locations was a potential risk for infants. The supervision should be strengthened to prevent the foodborne diseases.
出处
《中国食品卫生杂志》
2015年第6期607-610,共4页
Chinese Journal of Food Hygiene
基金
江西省卫生厅科研项目(20156016)
