异丙酚和七氟醚对人乳腺癌细胞转移能力的影响

Effects of propofol and sevoflurane on metastasis of human breast cancer cells

目的 评价异丙酚和七氟醚对人乳腺癌细胞转移能力的影响.方法 将人乳腺癌MDA-MB-231细胞以5×105个/ml的细胞密度（1 ml/孔）接种于6孔板,培养24 h后,采用随机数字表法,将培养孔分为3组（n=18）：对照组（C组）、异丙酚组（P组）和七氟醚组（S组）.P组给予异丙酚,终浓度5 μg/ml,同时通入95％O2-5％CO2,S组通入3.4％ ～ 3.5％七氟醚-5％CO2-O2,C组通入95％O2-5％CO2,气体流量均为1 L/min.各组均孵育6h,孵育结束后继续常规培养24 h,采用Transwell法检测侵袭细胞数,采用细胞划痕法检测细胞迁移率,采用Western blot法检测基质金属蛋白酶-9（MMP-9）表达.结果 与C组比较,P组和S组侵袭细胞数和细胞迁移率降低,MMP-9表达下调（P＜0.05）;P组和S组各指标比较差异无统计学意义（P＞0.05）.结论 异丙酚和七氟醚均可抑制人乳腺癌细胞的转移能力,且二者抑制作用无差异,其机制可能与下调MMP-9表达有关.

Objective To evaluate the effects of propofol and sevoflurane on metastasis of human breast cancer cells.Methods Human breast cancer cell line MDA-MB-231 cells were inoculated in 6-well culture plates at a density of 5× 105 cells/ml （1 ml/well）.After being cultured for 24 h, the cells were divided into 3 groups by using a random number table： control group （group C）, propofol group （group P）and sevoflurane group （group S）.In group P, propofol was given with a final concentration of 5 μg/ml, and the cells were simultaneously exposed to 95% O2-5% CO2 at 1 L/min.In group S, the cells were exposed to 3.4%-3.5%sevoflurane-5%CO2-O2 at 1 L/min.In group C, the cells were exposed to 95%O25%CO2 at 1 L/min.The cells were incubated for 6 h in each group.After the end of incubation, the cells were cultured routinely for 24 h.The invasion of cells was determined by Transwell invasion assay, and the invaded cells were counted.The migration of cells was determined by cell scratch test, and cell migration rates were calculated.The expression of matrix metalloproteinases-9 （MMP-9） was determined by Western blot.Results Compared with group C, the invaded cells and cell migration rates were significantly decreased, and the expression of MMP-9 was down-regulated in P and S groups （P〈0.05）.There was no significant difference in each parameter between group P and group S （P〉 0.05）.Conclusion Both propofol and sevoflurane can inhibt metastasis of human breast cancer cells, the inhibitory effect is similar between the two drugs, and the mechanism may be related to down-regulated expression of MMP-9.