老龄大鼠术后海马occludin和claudin-5表达的变化

Changes in expression of hippocampal occludin and claudin-5 after operation in aged rats

目的 探讨老龄大鼠术后海马occludin和claudin-5表达的变化.方法 健康雄性Wistar大鼠81只,16～18月龄,体重400～ 500 g,采用随机数字表法,将其分为3组（n=27）：正常对照组（C组）、切皮组（I组）和脾切除术组（S组）.S组制备脾切除术模型,I组仅进行切皮与缝合.分别于术前（T0）、术后1、3、7d（T1,3,7）时进行Morris水迷宫实验,水迷宫实验结束后,每组取9只大鼠,分离海马,采用Western blot法检测occludin和claudin-5的表达.结果 与C组比较,S组T13时逃避潜伏期延长,靶象限活动时间百分比降低,海马高磷酸化occludin和claudin-5表达下调,海马低磷酸化occludin表达上调（P＜0.05）.结论 老龄大鼠术后认知功能障碍的机制与海马高磷酸化occludin及claudin-5表达下调有关.

Objective To investigate the changes in the expression of hippocampal occludin and claudin-5 after operation in aged rats.Methods Eighty-one healthy male Wistar rats, aged 16-18 months, weighing 400-500 g, were randomly assigned into 3 groups （n =27 each） using a random number table： normal control group （group C）, skin incision group （group I） and splenectomy group （group S）.Splenectomy was performed in group S.Only skin incision and closing were performed in group I.Morris water maze test was performed before operation and on 1, 3 and 7 days after operation.The escape latency and the percentage of time spent at the target platform quadrant were recorded.Nine rats randomly selected from each group were sacrificed after the end of the test, and the hippocampi were isolated to detect the expression of occludin and claudin-5 by Western blot.Results Compared with group C, the escape latency was significantly prolonged, the percentage of time spent at the target platform quadrant was decreased, the expression of hyperphosphorylated occludin and claudin-5 was down-regulated, and the expression of hypophosphorylated occludin was up-regulated on 1 and 3 days after operation in group S （P〈0.05）.Conclusion The mechanism of postoperative cognitive dysfunction is related to the downregulated expression of hippocampal hyperphosphorylated occludin and claudin-5 in aged rats.