血液灌流对家兔毒死蜱清除能力的影响

The rabbit experimental study for toxicokinetics of chlorpyrifos impacted by hemoperfusion

目的 探讨血液灌流对毒死蜱家兔经口染毒代谢动力学参数的影响,探索血液灌流对毒死蜱的清除能力.方法 12只家兔以300 mg/kg毒死蜱灌胃染毒,然后按随机数字表法随机分为2组,每组6只.中毒模型组（对照组）染毒后不予血液灌流;血液灌流组染毒后0.5 h进行血液灌流,持续2h.血液灌流开始前及开始后5、15、30、45 min及1、1.5、2、4、8、12、24、48和72 h采集动脉血1 ml,气相色谱法检测血浆中毒死蜱含量,应用DAS3.0软件计算两组动物的毒物代谢动力学参数.结果 CPF在吸收入血后迅速分布到其他组织,然后缓慢代谢排出体外,CPF在家兔体内的代谢过程符合二室模型的特征.血液灌流组毒死蜱达峰时间为（7.19±3.74）h,峰浓度为（1.37±0.56） mg/L,清除率为（13.93±10.27）L/h/kg,表观分布容积为（418.18±147.15）L/kg,与对照组比较,差异有统计学意义（P＜0.05）.结论 血液灌流可以降低毒死蜱在家兔体内的负荷量,减少内暴露水平。

Objective To investigate toxicokinetic parameters impacted by hemoperfusion after oral chlorpyrifos exposure,to investigate the adsorption effect of hemoperhusion for chlorpyrifos poisoning.Methods 12 rabbits were divided into two groups after oral exposure with chlorpyrifos 300 mg/kg body weight.Control group：without hemoperfusion;hemoperfusion group：hemoperfusion starts 0.5 h after chlorpyrifos exposure and lasts for 2h.Blood samples were collected at different times,concentrations of chlorpyrifos were tested by GC,then,toxicokinetic parameterswere calculated and analysis by DAS3.0.Results In hemoperfusion group,peak time was （7.19±3.74） h,peak concentrations was （1.37±0.56） mg/L,clearance rate was （13.93± 10.27） L/h/kg,apparent volume of distribution was （418.18±147.15） L/kg The difference of these parameter were statistically significant compared with control group （P〈0.05）.Conclusion Hmoperfusion will decrease the inner exposure and load dose of rabbits with chlorpyrifos poisoning.