抗原加工相关转运体1在前列腺癌组织中的表达差异

The expression of transporter associated with antigen processing 1 in the prostate cancer

目的观察不同临床分期前列腺癌的抗原提呈路径的表达差异，探讨抗原加工相关转运体1（TAPl）与肿瘤发生及恶性程度相关性。方法收集前列腺癌、正常前列腺组织以及前列腺增生症（BPH）标本，进行Westernblot和免疫组织化学染色，并根据蛋白条带的灰度值和免疫组织化学结果进行定量评分。结果Westernblot显示前列腺癌标本中TAP1表达灰度为0．210±0．093，比较正常前列腺组（0．310±0．021）和BPH组（0．250±0．067），差异有统计学意义（P〈0．05）。免疫组织化学显示前列腺癌中TAP1阳性染色评分为（1．200±0．316）分，比较正常前列腺组的（2．600±0．244）分和BPH组的（2．000±0．235）分，差异有统计学意义（P〈0．05），且TAP1表达与前列腺痛的分期和Gleason评分相关。结论检测TAPl的表达有助于判断前列腺癌的预后。

Objective To explore the transporter associated with antigen processing 1 （TAP1） ex- pression differences in different clinical staging of prostate cancer. Methods The prostate cancer speci- mens, the normal prostate tissue and benign prostatic hyperplasia （BPH） specimens were obtained. The TAP1 expression was detected by Western blotting and immunohistochemical staining. According to the re- sults of immunohistochemical staining, the scores were calucated for the analysis of TAP1 expression. Re- suits According to the Western blotting results of TAP1, the TAP1 expression （0. 210 -±0. 093） in pros- tate cancer was lower than that in normal prostate （0. 310 ±0. 021 ） and BPH （0. 250 ± 0. 067）. The TAP1 score of immunohistochemical staining （1. 200 ± 0. 316） in prostate cancer was lower than that in normal prostate （2. 600 ± 0. 244） and BPH （ 2. 000 ± 0. 235 ）. The TAP1 expression was also correlated with tumor stage and Gleason scores. Conclusion Detection of TAP1 expression can provide evidience of prognosis for patients with prostate cancer.