褪黑素、钙调蛋白在脊柱侧凸模型中的相互作用

Effect of melatonin and calmodulin on idiopathic scoliosis model

目的观察持续光照、褪黑素拈抗剂、钙调蛋白拮抗剂共同作用对制作双足大鼠脊柱侧凸动物模型成功率及Cobb角度的影响。方法将刚断乳的1个月龄雌性大鼠32只制作成双足大鼠，并随机分成4组，A组予腹腔注射褪黑素拮抗剂luzindole＋持续光照，B组单纯予腹腔注射褪黑素拮抗剂luzindole，C组予腹腔注射褪黑素拮抗剂luzindole＋饮用钙调蛋白拮抗剂TMX，D组予腹腔注射等量生理盐水作为空白对照。8周和16周时分别拍摄x线片并统计各组大鼠侧凸模型发生率和侧凸程度的不同。结果（1）8周时，AB两组大鼠部分发生了脊柱侧凸，发生率分别为75．0％和12．5％，其中A组侧凸度数范围分别为10．8°～16．8°，平均为12．4°，B组大鼠侧凸度数平均为19．4°，AB组大鼠侧凸发生率差异有统计学意义（P〈0．05），CD两组大鼠均没有发生脊柱侧凸，未发生率为100．0％；（2）16周时，A组大鼠脊柱侧凸的发生率为57．0％，侧凸度数范围10．1°～17．9°，平均14．3°，B组大鼠脊柱侧凸发生率为62．5％，侧凸度数范围18．0°～30．3°，平均25．2°，A、B组大鼠侧凸发生率差异无统计学意义（P〉0．05）。CD两组大鼠均未发生脊柱侧凸，未发生率为100．0％，BC、BD组大鼠侧凸发生率差异有统计学意义（P〈0．05）。结论（1）通过在双足大鼠腹腔注射褪黑素拮抗剂，可成功制作脊柱侧凸大鼠模型，这种脊柱侧凸的发生与褪黑素的失作用有关。（2）光照条件下，早期可增加脊柱侧凸的发生，但是这种侧凸是可逆的。（3）光照条件最终不能增加脊柱侧凸的发生率及侧凸畸形程度。（4）钙调蛋白拮抗剂可有效抑制脊柱侧凸的自然进程，这种抑制作用与钙调蛋白的失作用有关。

Objective To explore influence of continuous illumination, luzindole and calmodulin antagonist, on success rate and Cobb angle of making animal model of bipedal rat scoliosis. Methods Make 32 one - month female rats, who were just weaned, into bipedal rats and randomly divided them into 4 groups. Group A： luzindole by intraperitoneal injection ＋ continuous illumination, Group B： luzindole by intraperitoneal injection, Group C： luzindole by intraperitoneal injection ＋ calmodulin antagonist TMX by drinking and Group D： equivalent normal saline by intraperitoneal injection as blank control. Take X - ray films on weeks 8 and 16 and count scoliosis model incidence and different scoliosis degree of different groups of rats. Results （ 1 ） On week 8, scoliosis occurs in part of rats in groups A and B, with incidence of 75.0% and 12. 5% respectively. Scoliosis degrees of group A are between 10. 8~ and 16. 8~, with an average of 12.4~, and average scoliosis of group B is 19. 4°. The scoliosis incidences of both groups are with statistically significant differences （ P 〈 0. 05 ）. Either group C or D has no seoliosis, with incidence of 0 ; （2） On week 16, scoliosis incidences of groups A and B are 57.0% and 62. 5 % respectively, and de- grees of which are between 10. 1° and 17.9° for group A and between 18.0° and 30. 3° for group B, with an average of 14. 3° and 25.2° respectively. Scoliosis incidences of groups A and B are of no significant difference （ P 〉 O. 05 ）. No scoliosis occurs in either group C or group D, with incidence of 0, while inci- dences of groups B and C as well as groups B and D are of significant differences （ P 〈 0. 05 ）. Conclusion （1） By intraperitoneal injection of luzindole to bipedal rats, scoliosis rat models could be successfully made, the incidence of scoliosis is associated with the loss of effect of melatonin ; （2） Under light, the oc- currence of scoliosis may be increased in early period, but it is reversible; （3） Light conditions can not in- crease the incidence and malformation of scoliosis; （4） Calmodulin antagonists can suppress the natural process of scoliosis, and the suppression is associated with the loss of effect of calmodulin.