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一种发掘ε-聚赖氨酸合成酶的生物信息学新方法

A new method to in silico identify ε-poly-L-lysine synthetases
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摘要 ε-聚赖氨酸（ε-Poly—L—lysine，ε-PL）作为一种天然防腐剂，已知的ε-PL产生菌基本仅在链霉菌科（Streptomycetaceae）麦角菌真菌（Ergot fungi）中发现。目前ε-PL产生菌筛选方法仍为传统的化学方法，存在工作量大、周期长等劣势，所用染料存在一定毒性。
作者 金城
机构地区 <微生物学通报>编委会
出处 《微生物学通报》 CAS CSCD 北大核心 2015年第12期2494-2494,共1页 Microbiology China
关键词 Ε-聚赖氨酸 ε-聚赖氨酸合成酶 生物信息学识别 ε-poly-L-lysine, ε-poly-L-lysine synthetase, in silico identification
分类号 Q814 [生物学—生物工程]
  • 相关文献

参考文献3

  • 1Nishikawa M, Ogawa K. Distribution of microbes producing antirnicrobial e-poly-L-lysine polymers in soil microflora determined by a novel method[J]. Applied and Environmental Microbiology, 2002, 68(7): 3575-3581.
  • 2Geng WT, Yang C, Gu YY, et al. Cloning of epsilon-poly-L-lysine (e-PL) synthetase gene from a newly isolated e-PL-producing Streptomyces albulus NK660 and its heterologous expression in Streptomyces lividans[J]. Microbial Biotechnology, 2014, 7(2): 155-164.
  • 3王静,谭之磊,毕德玺,贾士儒,欧竑宇.细菌基因组序列中ε-聚赖氨酸合成酶的生物信息学识别与分析[J].微生物学通报,2015,42(12):2495-2504. 被引量：3

二级参考文献20

  • 1Hyldgaard M, Mygind T, Vad BS, et al. The antimicrobial mechanism of action of epsilon-poly-L-lysine[J]. Applied and Environmental Microbiology, 2014, 80(24): 7758-7770.
  • 2Shima S, Matsuoka H, Iwamoto T, et al. Antimicrobial action of ε-poly-L-lysine[J]. The Journal of Antibiotics, 1984, 37(11): 1449-1455.
  • 3Hamano Y. Occurrence, biosynthesis, biodegradation, and industrial and medical applications of a naturally occurring ε-poly-L-lysine[J]. Bioscience, Biotechnology, and Biochemistry, 2011, 75(7): 1226-1233.
  • 4Nishikawa M, Ogawa K. Distribution of microbes producing antimicrobial ε-poly-L-lysine polymers in soil microflora determined by a novel method[J]. Applied and Environmental Microbiology, 2002, 68(7): 3575-3581.
  • 5Geng WT, Yang C, Gu YY, et al. Cloning of epsilon-poly-L-lysine (ε-PL) synthetase gene from a newly isolated e-PL-producing Streptomyces albulus NK660 and its heterologous expression in Streptomyces lividans[J]. Microbial Biotechnology, 2014, 7(2): 155-164.
  • 6Gu YY, Yang C, wang XM, et al. Genome sequence of the ε-poly-L-lysine-producing strain Streptomyces albulus NK660, isolated from soil in Gutian, Fujian province, China[J]. Genome Announcements, 2014, 2(3): e00532-14.
  • 7Nishikawa M, Kobayashi K. Streptomyces roseoverticillatus produces two different poly(amino acid)s: lariat-shaped gamma-poly(L-glutamic acid) and epsilon-poly(L-lysine)[J]. Microbiology, 2009, 155(Pt 9): 2988-2993.
  • 8Ouyang J, Xu H, Li S, et al. Production of ε-poly-L-lysine by newly isolated Kitasatospora sp. PL6-3[J]. Biotechnology Journal, 2006, 1(12): 1459-1463.
  • 9Xu Z, Xia J, Feng X, et al. Genome sequence of Streptomyces albulus PD-1, a productive strain for epsilon-poly-L-lysine and poly-L-diaminopropionic acid[J]. Genome Announcements, 2014, 2(2): e00297-14.
  • 10Hamano Y, Kito N, Kita A, et al. ε-poly-L-lysine peptide chain length regulated by the linkers connecting the transmembrane domains of ε-poly-L-lysine synthetase[J]. Applied and Environmental Microbiology, 2014, 80(16): 4993-5000.

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微生物学通报
2015年 第12期

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