乌头碱配伍人参皂苷Rb_1对原代培养心肌细胞能量代谢的影响

Compatibility Effect of Aconitine and Ginsenosides Rb_1 on Energy Metabolism of Primary Cultured Myocardial Cells

目的:探讨乌头碱配伍人参皂苷Rb1对心肌细胞能量代谢的影响。方法:采用胰蛋白酶消化和差速贴壁法培养大鼠乳鼠心肌细胞,分为正常对照组、0.2%乌头碱组、0.1%乌头碱组、人参皂苷Rb1组、乌头碱配伍人参皂苷Rb1组(1?1、1?2、2?1),给药作用1 h,检测心肌细胞的细胞活力和体内糖原、琥珀酸脱氢酶、细胞色素C氧化酶以及细胞乳酸脱氢酶的含量。结果:0.2%乌头碱能够明显降低心肌细胞活力,增加乳酸脱氢酶含量,降低细胞内糖原、琥珀酸脱氢酶、细胞色素C氧化酶含量;1×10-6 mol·L-1或2×10-6 mol·L-1浓度的人参皂苷Rb1配伍乌头碱,可明显对抗0.2%乌头碱对心肌细胞的毒性。结论:人参皂苷Rb1可降低乌头碱心肌细胞毒性,其作用机制可能与对抗细胞能量代谢有关。

This article was aimed to discuss the impact of compatibility effect of aconitine and ginsenosides Rb1 on energy metabolism of cardiomyocytes. The trypsin was used to digest and differential adherence method was used to purify and cultivate cardiomyocytes. Rat cardiomyocytes were divided into the normal control group, 0.2% aconitine group, 0.1% aconitine group, ginsenosides Rb1 group, compatibility of aconitine and ginsenosides Rb1（1：1, 1：2, 2：1） group. The drug reaction time was 1 hour. The cell vitality, glycogen content, succinate dehydrogenase, cytochrome C oxidase and LDH concentration were detected in cardiomyocytes. The results showed that 0.2% aconitine significantly reduced myocardial viability, increased the LDH concentration, reduced the glycogen content, succinate dehydrogenase and cytochrome C oxidase. The compatibility of 1×10-6 mol·L-1 or 2×10-6 mol·L-1 ginsenosides Rb1 and aconitine was significantly against the cardiomyocytes toxicity induced by 0.2% aconitine. It was concluded that ginsenosides Rb1 can reduce the cardiomyocytes toxicity by aconitine. Its mechanism may be associated with the cellular energy metabolism.