金莲花药渣发酵提取物拮抗白色念珠菌的体内外实验研究

Effects of Globeflower Residue Fermentation Extraction on Anti-Candida albicans in vivo and in vitro

目的:观察金莲花药渣发酵提取物(Extraction of Globeflower Residue Fermentation,EGRF)体外对白色念珠菌抑杀效果及体内对小鼠念珠菌性阴道炎的治疗效果。方法:通过试管法配合平皿法检测EGRF对白色念珠菌的最小抑菌浓度(Minimum Inhibitory Concentration,MIC)和最小杀菌浓度(Minimum Bactericidal Concentration,MBC);构建小鼠白念珠菌性阴道炎模型,将造模成功小鼠随机分为正常对照组,模型组,EGRF低、中、高剂量组(EGRF给药浓度分别为:40、80、160 mg?kg-1),氟康唑组(20 mg?kg-1),共6组。各药均阴道内给药,每天1次,连续给药7天,观察小鼠外阴炎症反应程度、阴道念珠菌转阴率、阴道灌洗液载菌量和阴道粘膜的病理改变。结果:EGRF对白色念珠菌的MIC为0.31 mg?m L-1,对白色念珠菌的MBC为1.25 mg?m L-1,EGRF和氟康唑的效价单位比MIC为2?1,MBC为1?1。与模型组相比,EGRF及氟康唑治疗组的小鼠外阴炎症反应程度减轻及分泌物中念珠菌数量随着给药时间增加而显著减少;小鼠阴道灌洗液白色念珠菌转阴率增加,载菌量明显减少;小鼠阴道组织病理改变有不同程度的减轻,有明显剂量依赖性,高剂量组与氟康唑组相近。结论:EGRF于体外对白色念珠菌抑杀作用显著,对小鼠白色念珠菌性阴道炎疗效较好。

This study was aimed to observe the antibacterial activity and bactericidal action of C.albicans in vitro, and the effects of curing monilial vaginitis mouse by extraction of globeflower residue fermentation（EGRF） in vivo. In vitro study, test tube method as well as plate method were used to determine the minimum inhibitory concentration（MIC） and minimal bactericidal concentration（MBC） of C.albicans respectively. In vivo, mouse were devided into normal controlled group, C. albicans vaginitis model group（Model）, Model ＋ EGRF（40, 80, 160 mg·kg-1） group, and fluconazole group（20 mg·kg-1）. All drugs were vaginal delivery once a day with continuous administration for seven days. Then vulva inflammation, negative rate of vaginal discharge, microbial load of vaginal lavage and the pathological changes of vaginal mucosa were observed. After the treatment of EGRF, the MIC and MBC of Candida albicans were 0.31 mg·m L-1 and 1.25 mg·m L-1, respectively, while the potency unit ratios between EGRF and fluconazole of MIC and MBC were 2 to 1 and 1 to 1, respectively. In comparison with Model, vulva inflammation of Model ＋ EGRF gourp and fluconazole group was improved, while C. albicans count in vaginal secretions of these two groups were decreased, the overcast rate of C. albicans of vaginal douche was increased, and pathological changes of vaginal mucosa were also improved in the two groups, which were in dose-dependent manners. And high dose Model ＋ EGRF group was close to fluconazole group. In conclusion, EGRF had obvious inhibitory effect on C. albicans in vitro. It also had a better therapeutic effect on C. albicans vaginitis mouse.