不同香型茶树鲜叶挥发性组分与β-葡萄糖苷酶的相关性分析

Relationship Between Volatile Constituents and β-glucosidase of Different Tea Varieties

为了明确不同香型茶树(Camellia sinensis)品种鲜叶挥发性物质的组成、含量、β-葡萄糖苷酶活性及其基因差异表达特征,以黄观音、0318E、福云6号、0213-2和C19五个四年生茶树品种(系)为实验材料,使用GC-MS测定其鲜叶挥发性物质的组成及含量,并对β-葡萄糖苷酶活性进行测定,同时克隆β-葡萄糖苷酶基因,分析其在不同品种中的表达差异。结果表明,黄观音和0318E的鲜叶中,萜烯类与衍生物种类及含量都高于普通香型茶树鲜叶(福云6号、0213-2和C19);鲜叶中β-葡萄糖苷酶活性以黄观音(7.8 U·g–1)和0318E(7.3 U·g–1)最高,福云6号和C19次之,0213-2(6.1 U·g–1)最低。β-葡萄糖苷酶基因在黄观音和0318E中表达最强,显著高于C19、福云6号和0213-2,在0213-2中的表达量最低。

We aimed to investigate the constituents and content of volatile compounds and the activity and differential gene expression of β-glucosidase among 4-year-old tea varieties（Camellia sinensis）（Huangguanyin, 0318 E, Fuyun 6, 0213-2 and C19） with different flavor properties. The volatile compounds of the tea varieties were determined by GC/MS. β-glucosidase activity was also measured. The expression of β-glucosidase in tea varieties was analyzed by quantitative RT-PCR. The content of terpenoids and its derivatives was greater in Huangguanyin and 0318 E than Fuyun 6, 0213-2 and C19. The β-glucosidase activity of Huangguanyin and 0318 E tea varieties was higher than other tea varieties, and the β-glucosidase activity of 0213-2 tea variety was the lowest. The expression of β-glucosidase was higher in Huangguanyin and 0318 E than in other samples, but 0213-2 had the lowest expression.