遗传与环境交互作用对紧张反应易感性影响

Interactive effect of genetic and environmental factors on stress response susceptibility

目的了解遗传与环境因素的交互作用对紧张反应易感性的影响。方法采用单纯病例研究设计,按照整群随机抽样方法抽取工作紧张测量量表(JSS)得分>90%正常参考值的323名汉族职员进行量表调查,采用Snapshot技术检测GCCR基因4个位点:Rs10482605、Rs41423247、Rs258747、Rs17209237,完成基因-环境交互作用的分析。结果 323名JSS得分较高的脑力作业者中,GCCR基因rs10482605突变型组工作紧张均分为(3.34±0.09)分,野生型组为(3.28±0.07)分,突变型组高于野生型组(P<0.05);GCCR基因rs17209237突变型组在生活事件总刺激项上均分为(49.41±15.60)分,高于野生型组均分(45.76±15.77)分(P<0.05);GCCR基因rs41423247突变型与主观支持有负相乘交互作用(P<0.05,OR=0.237)。结论 GCCR基因可能是紧张反应的遗传易感因素,且与环境因素(社会支持)存在交互作用。

Objective To investigate the interactive effect of genetic and environmental factors on the susceptibility to stress response among white-collar workers. Methods With stratified cluster sampling, we recruited 323 persons with scores of Job Stress Survey（JSS） higher than 90% of the normal reference value derived from a screening survey on occupational stress with JSS among 3 200 Han white-collar workers. All the participants were surveyed with Life Event Scale（ LES ） and Social Support Rating Scale, and their blood samples were collected and genotyped for 4 locus of glucocorticoid receptor gene（GCCR：Rs10482605,Rs41423247,Rs258747,Rs17209237） with Snapshot technology. Results The participants with the mutation type of GCCR rs10482605 had a significantly higher average JSS score than those with the wild type （3.34 ±0. 09 vs. 3.28 ± 0. 07, P 〈 0. 05 ） ;the participants with the mutation type of GCCR rs17209237 had a significantly higher overall LES score than those with the wild type（49.41 ± 15.60 vs. 45.76 ± 15.77 ,P 〈0. 05）. The mutated GCCR rs41423247 presented a negative multiplicative interaction to subjective social support（ odds ratio = 0. 237,P 〈 0. 05）. Conclusion Gene GCCR may be a genetic risk factor for stress response and may have interactive effect with environmental factors on stress response in human.