肝细胞癌中CD90抗原的表达及与上皮间质转化的关系

Expression of tumor CD90 in hepatocellular carcinoma and its relationship with epithelial mesenchymal transition

目的探讨肝细胞癌中干细胞相关蛋白CD90的表达和临床病理学特征的关系，分析CD90蛋白和上皮间质转化相关蛋白上皮钙黏素（E—cad）、神经钙黏素（N—cad）表达的关系。方法采用免疫组织化学SP法检测53份原发性肝细胞癌组织中CD90、E—cad、N—cad的表达，并以10份肝良性病变周围肝组织作对照。应用流式细胞技术检测MHCC97-L和HCCLM一3两个肝癌细胞系CDg0阳性细胞中E—cad、N—cad蛋白的表达情况。计数资料采用卡方检验，Spearman等级相关分析比较。计量资料采用独立样本t检验进行比较。结果对照肝组织中无CD90表达，肝细胞癌组织中CD90蛋白阳性率为34％（18／53），两组差异有统计学意义（X2=4．755，P〈0．05）。肝细胞癌组织中CD90蛋白表达和门静脉癌栓呈正相关（r=0．378，P〈0．05），与组织分化和包膜浸润呈负相关（r=-0．398、-0．519，P均〈0．05）。肝细胞癌组织中CD90蛋白表达与E—cad表达呈负相关（r=-0．341，P〈0．05），与Ncad表达呈正相关（r=0．441，P〈0．05）。HCCLM-3细胞系中CD90阳性细胞的E-cad蛋白表达低于MHCC97-L细胞系[（2．56±0．29）％和（4．31±0．18）％，t=8．757，P〈0．051，而N—cad蛋白表达高于MHCC97-L细胞系[（8．10±1．45）％和（5．51±0．44）％，t=-9．667，P〈0．051。结论肝细胞癌干细胞相关蛋白CD90的表达与肿瘤发生上皮间质转化存在相关性，两者相互作用促进肿瘤侵袭转移，可能是肝细胞癌治疗的一个新靶点。

Objective To investigate the expression of stem cell associated protein CD90 in primary hepatocellular carcinoma （HCC） and its relationship with clinical pathological characters; to analyze the relation between CD90 and epithelial mesenchymal transition （EMT） markers E-cadherin （E cad） and N cadherin （N-cad）. Methods The expressions of CD90, E-cad and N-cad in 53 tissues of HCC were detected by immunohistochemistry streptavidin-perosidase （SP） method, and 10 surrounding of liver benign lesions were studied as control. The expression of E-cad and N-cad in CD90 positive HCC cell lines （MHCC97-L and HCCLM-3） was measured by flow cytometry. Chi cells of two re test and Spearman rank correlation analysis were performed for count data. Independent sample t test was used for measurement data comparison. Results There was no CD90 expression in control liver tissue. The positive rate of CD90 expression in HCC tissue was （34%, 18/53）, and the difference between two groups was statistically significant （X2 = 4. 755, P〈0. 05）. The expression of CD90 in HCC was positively correlated with portal vein cancer embolus （r=0. 378, P〈0. 05） and was negatively correlated with histological differentiation degree and capsular infiltration （r=- 0. 398 and -0.519, both P〈0.05）. The expression of CD90 was negatively correlated with the expression of E-cad （r=-0. 341, P%0.05）, however was positively correlated with the expression of N-cad （r=-0. 441, P〈0.05）. The positive expression rate of E- cad in CD90 positive HCCLM-3 cells was lower than that of MHCC97-L cells （（2. 56±0. 29）% and （4.31±0.18）% ,t- 8. 757, P〈 0. 05）, while the positive expression rate of N-cad was higher than that of MHCC97 L cells （（8.10±1.45）% and （5.51±0.44）% ,t= -9. 667,P〈0.05）. Canelusians The expression of stem cell associated protein CD90 is correlated with the EMT of HCC. Their interaction promote tumor infiltration metastasis which may be a new target of HCC treatment.