全自动检测和分选全血中白细胞的微流控芯片装置研究

Automatic Detecting and Sorting White Blood Cells from the Whole Blood Sample via a Microfluidic Chip Device

在生物学和医学研究中从全血中分离和纯化白细胞有着广泛的需求。该文报道了一种可全自动、连续从全血中检测和分离白细胞的微流控芯片装置。样品中的每个细胞流经微通道中的阻抗脉冲检测区时,均会产生一个电阻脉冲信号(resistive pulse sensing,RPS)并且其幅值大小表示细胞的粒径大小。当白细胞通过检测区时,会产生幅值较大的信号,Labview程序对采集到的RPS信号进行分析、处理并自动触发电动分选过程,从而将白细胞分至目标通道,而全血中的红细胞则流至废液池。该微流控芯片装置具有系统简单、全自动操作和目标细胞无标记等特点,有望发展成为便携式白细胞分选装置。

There is increasing demand on the white blood cells sorted from the whole blood sample in the biological and medical researches. We reports a microfluidic lab on chip device by which the white blood cells can be automatically and continually detected and sorted from the whole blood cells. While each cell of the sample solution passes through the resistive pulse sensing gate, a resistive signal pulse is generated. The magnitude of the signal represents the size of the cell. For the white blood cell which is larger in volume, a signal with larger magnitude is generated. The Labview program acquires the RPS signals and triggers the cell sorting process once a white blood cell is detected, thus sorting the white blood cell into the target channel. For the red blood cells, they are transported to the waste reservoir. The device described here is simple, automatic and label-free. It has the potential to become a portable device for white blood cell sorting.