吴茱萸次碱对三种肿瘤细胞体外增殖抑制作用的研究

Anti-proliferative effects of rutaecarpine on three tumor cells

目的:探讨吴茱萸次碱的体外抗肿瘤活性。方法:采用MTT法检测了不同浓度的吴茱萸次碱在不同的时间点对S180肉瘤细胞、H22肝癌细胞、人源Hep G2肝癌细胞的抑制情况。结果:吴茱萸次碱在浓度为64、32、16、8、4μg/ml时,对S180肉瘤细胞、H22肝癌细胞、人源Hep G2肝癌细胞均具有明显的抑制作用,随着药物浓度增加,抑制率逐渐增大,与阴性对照组比较作用极其显著。吴茱萸次碱作用细胞24h时,对三种肿瘤细胞的半数抑制浓度(IC50)分别为34.10、24.81和28.07μg/ml,对H22的IC50值最小(24.81μg/ml),生长抑制作用最好;作用48h时,对三种肿瘤细胞的半数抑制浓度(IC50)分别为19.35、20.62和27.81μg/ml,对S180的IC50值最小(19.35μg/ml),抑制作用相对最好;作用72h时,对三种肿瘤细胞的半数抑制浓度(IC50)分别为15.35、17.74和14.52μg/ml,对Hep G2的IC50值最小(14.52μg/ml),抑制作用最好。结论 :吴茱萸次碱对三种肿瘤细胞增殖均有明显的抑制作用。

Objective： The study of antitumor activity of rutaecarpine alkaloid in vitro. Methods： The inhibition effects of rutaecarpine at different concentrations after 24 h,48h,72 h against S180,H22,Hep G2 tumor cells growth in vitro were investigated by MTT assay. Results： The results indicated rutaecarpine could obviously inhibit the growth of S180,H22,Hep G2 tumor cells. With the increasing of drug concentrations,the inhibition rate of rutaecarpine against the three tumor cells gradually increased. Furthermore,the inhibition rate of each group had extremely significant effect as compared with controls. The IC50 values shown rutaecarpine to the H22 cells were the most sensitive in the three tumor cells in 24 h. However,in 48 h,S180 were more sensitive than H22 and Hep G2 cells. And in 72 h,Hep G2 tumor cells were the most sensitive（ IC50= 14. 52μg / ml）. Conclusion： Rutaecarpine could obviously inhibit the growth of S180,H22,Hep G2 tumor cells.