氧化剂抑制白色假丝酵母菌菌丝形成的研究

Experimental study on oxidants function in inhibiting hyphae of Candida albicans

目的研究3种氧化剂对白色假丝酵母菌(CAL)菌丝形成的影响,探讨氧化应激对真菌病原体的抑制作用,为研发新的抗真菌药物提供理论依据。方法 2014年2月将3株CAL标准株和临床分离菌株悬液接种于含不同浓度过氧化氢、二酰胺和甲萘醌的RPMI-1640培养基中,分别于不同时间(0、2、4、6h)计数菌丝生成率,数据采用SPSS 13.0软件进行统计分析。结果 3种氧化剂对CAL菌丝形成均有抑制作用,H2O2、二酰胺和甲萘醌均呈剂量依赖性抑制菌丝生成,H2O2、二酰胺和甲萘醌浓度为30mmol/L时,CAL菌丝生成率开始下降,菌丝生成率分别为67.0%、58.0%、49.0%;在H2O2、二酰胺和甲萘醌处理2h时,CAL菌丝生成率开始下降,生成率分别为73.0%、59.0%、51.0%。结论 3种氧化剂呈浓度和时间依赖性抑制CAL菌丝形成,其中甲萘醌抑制CAL菌丝形成的作用最强,并且标准株菌丝生成率低于临床分离株。

OBJECTIVE To observe the effect of three kinds of oxidants on inhibiting hyphae formation of C. albicans and explore oxidative stress inhibition of fungal pathogens so as to provide a theoretical basis for the develop- ment of new agents. METHODS In Feb. 2014, 3 C. albicans standard strains and isolated strains obtained from clinical were inoculated in RPMI-1640 culture mediums containing different concentrations of hydrogen peroxide, diamide, and menadione, and the generate rate of mycelium at different times were counted （0, 2, 4, 6 h）. SPSS 13.0 was explored to make statistical analysis. RESULTS The results showed that compared with the control group, three kinds of oxidants inhibite hyphae formation of C. albicans. HzOz, diamide and menadione generated a dose-dependent inhibition of mycelium, and the formation rate of C. albicans hyphae （67. 0%, 58. 0K, and 49.0%） began to decline at concentration of 30mmol/L. In addition, H2Oz, diamide and menadione showed a time-dependent inhibition of mycelium generated and when C. albicans were exposed to Hz O2, diamide and menadione for 2 h, C. Albicans hyphae formation rates （73. 0%, 59. 0;, and 51. 0;） began to decline. CONCLUSION The three oxidants were concentration and time-dependent in inhibition of C. albicans hyphae formation. Menadione is the strongest on inhibition of C. albicans hyphae, and the production rate of the standard strains is lower than the clinical isolates.