氨基介孔磁性载体在柴油生物脱硫中的应用

Application of Amino Mesoporous Magnetic Carrier on Biological Desulfurization of Diesel Oil

制备了氨基介孔磁性载体(AMMC),分别采用FTIR,XRD,TEM等技术对AMMC的官能团、磁性、表观形态和沉降性能等进行了表征,并将脱硫菌株固定于AMMC上,以含二苯并噻吩(DBT)的正十六烷为模拟柴油,比较了固定化脱硫菌和游离脱硫菌对柴油的脱硫性能。表征结果显示,AMMC负载了氨基,是具有丰富孔结构的磁性载体(比表面积为61 m^2/g、孔体积为0.131 cm^3/g、平均孔径为11.339 nm),具有悬浮能力强、磁性良好、分离速度快的特点。脱硫实验结果表明:AMMC固定化脱硫菌配合吐温80使用,对DBT的降解率最高,脱硫反应第3天,DBT降解率为75%;AMMC固定化脱硫菌未经活化直接第5次重复使用时,DBT降解率维持在30%左右,游离脱硫菌的DBT降解率不足10%。

The amino mesoporous magnetic carrier （AMMC） for desulfurization bacteria immobilization was prepared and characterized by FTIR,XRD and TEM in aspects of functional group,magnetism,morphology and sedimentation performance,etc.. Using n-hexadecane containing dibenzothiophene as the simulated diesel oil,the desulfurization capability of the immobilized cells and free cells to diesel oil is compared with each other. The characterization results show that AMMC is a magnetic carrier with loaded amino-group and well-developed pore structure （the specific surface area is 61 m^2/g,the pore volume is 0.131 cm^3/g,the average pore size is 11.339 nm）,which has good properties of suspendability,magnetism and separation rate. The desulfurization experimental results show that：When the desulfurization bacteria immobilized on AMMC are used combining with Tween 80,the DBT degradation rate is the highest,and is 75% after reacted for 3 d;When the desulfurization bacteria immobilized on AMMC are reused at the 5th time and without activation,the DBT degradation rate is still about 30%,while that of the free cells is less than 10%.