溶氧控制对木聚糖酶发酵过程影响的研究

Study on the effect of dissolved oxygen control on xylanase fermentation process

溶氧是影响木聚糖酶发酵的重要因素之一。为了优化木聚糖酶发酵过程的溶氧控制,在30 L自动发酵罐上,考察了搅拌转速和通风比不同条件下的溶氧水平对木聚糖酶发酵的影响。结果表明,发酵前期通过搅拌转速自动控制溶氧,对数期溶氧控制在15%,后期溶氧控制在45%,在此条件下,木聚糖酶活高达2406.175 U/m L,比分段控氧前酶活提升了50%。本文提出了通过溶氧与搅拌联动并配合通风比的调节实现罐内分段控氧的新思路。

Dissolved oxygen was a key operational parameter during xylanase fermentation process. In order to optimize dissolved oxygen for xylanase production,the effect of dissolved oxygen change accordingly with agitation speed and ventilation rate was investigated in a 30 L automatic fermentor. On the basis,agita-tion speed and ventilation rate were adjusted to control the dissolved oxygen at different fermentation stages. The results showed that the optimized strategy was found that at the earlier fermentation period, the dissolved oxygen was automatically controlled,at logarithmic phase the dissolved oxygen was controlled to 15% and in fermentation anaphase the dissolved oxygen was controlled to 45%. Under this conditions,the xylanase activity reached 2406.175 U/m L,which was 50% higher than before.