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27重SNP种族推断体系准确性验证研究 被引量：14

The evaluation of the effectiveness and accuracy for a 27-plex SNP assay
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摘要 目的验证所构建27重SNP复合检测体系,用于东亚、欧洲、非洲及其混合人群种族推断的有效性和正确性。方法国内7家单位的实验室提供盲测样本316份,由本中心实验室利用构建的27重复合SNP检测体系,使用毛细管电泳对样本进行检测。结果 316份样本检测结果与样本的已知祖先来源的一致性为99.37%。结论该体系可以有效区分洲际人群的祖先来源,为案件侦查提供重要的科学线索。 Objective We constructed a 27-plex SNP assay for the inference of East Asian, European, African and mixture populations. In this paper, we evaluated the effectiveness and accuracy of the 27-plex SNP assay by blind test. Method We tested 316 individuals from 7 different labs of China using the 27-plex SNP assay. The SNP genotype can be assayed by capillary electrophoresis. Result 99. 37% of the prediction results are in concordance with the known ancestry of these samples. Conclusion The results demonstrated that the 27-plex SNP assay can be effectively used to analyze the genetic continental components of a population and infer the ancestral origin of a DNA sample. It can provide important scientific clues for case investigation.
作者 刘海渤 孙启凡 陈健刚 魏以梁 李越 唐晖 程宝文 李平 迪力.夏提 张涛 马泉 赵兴春 李彩霞
机构地区 公安部物证鉴定中心北京市现场物证检验工程技术研究中心 新疆生产建设兵团公安局 新疆自治区公安厅 天津医科大学 广州市刑事科学技术研究所 北京市公安局法医检验鉴定中心 云南省公安厅 喀什市公安局 克孜勒苏柯尔克孜自治州公安局
出处 《中国法医学杂志》 CSCD 2015年第6期563-566,共4页 Chinese Journal of Forensic Medicine
基金 北京市科技创新基地培育与发展工程项目(Z141106004414084) 公安部法医遗传学公安部重点实验室开放课题(2014FGKFKT04)
关键词 法医物证学 SNPS 复合扩增 种族推断 forensic evidence, SNPs, multiplex system, race inference
分类号 D919 [医药卫生—法医学]
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参考文献8

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同被引文献90

引证文献14

二级引证文献49

中国法医学杂志
2015年 第6期

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