超临界CO_2萃取苹果树枝根皮苷工艺研究

Study on techniques of phloridzin extraction from branches of apple tree by supercritical CO_2

为探索超临界CO_2萃取技术在苹果树枝根皮苷提取中的应用效果与最佳工艺条件,以冬季修剪时废弃的苹果树枝为试验材料,乙醇为夹带剂,在单因素试验的基础上通过正交试验对超临界CO_2萃取苹果树枝根皮苷工艺进行了优化。结果表明,在试验设计范围内,夹带剂用量对超临界CO_2提取苹果树枝根皮苷得率的影响最大,其次为萃取时间和萃取温度,以萃取压力的影响最小;超临界CO_2萃取苹果树枝中根皮苷的最佳工艺条件为:萃取温度40℃、萃取时间45min、萃取压力30MPa、夹带剂用量30m L,在此条件下根皮苷提取得率经验证试验可达到2.02%,提取物纯度达到31.94%,显著高于乙醇浸提法。应用超临界CO_2萃取技术提取苹果树枝中根皮苷,提取条件温和,时间短,提取物纯度高,是一种较好的根皮苷提取分离方法。

In order to elucidate the effect of applying supercritical CO2 extraction in isolation of phloridzin from branches of apple tree and optimize the extraction technique,the optimal condition for supercritical CO2 extraction of phloridzin from branches of apple tree was investigated by using orthogonal test with ethanol as co-solvent on the basis of single test.Results showed that among the four test factors,the co-solvent amount had the strongest influence on yield of phloridzi,and the extraction duration and temperature came after,while the extraction pressure had very little influence.The optimum condition for supercritical CO2 extraction of phloridzin from branches of apple tree was：temperature at 40℃,0 MPa is 30,co-solvent is 30 m L and duration is 45 min.The yield of phloridzin from branches of apple tree achieved 2.02% under the optimal extraction condition,and the purity of the extract was 31.94%,which was far superior to that of extraction with ethanol.Extraction of phloridzin by supercritical CO2 has high purity with mild conditions and short duration.These results suggest that supercritical CO2 extraction is a good method for isolating phloridzin from branches of apple tree.