三种中药组方对快速上浮脱险减压病大鼠肺组织炎症反应的影响

Effects of 3 Chinese medicinal drug formulas on the lung inflammatory response in the rats with decompression sickness induced by fast buoyancy ascent escape

目的 观察3种中药组方对快速上浮脱险减压病大鼠肺组织炎症反应的影响,筛选出疗效确切的药组.方法 100只实验大鼠分为5组：正常组、模型组、1号药组、2号药组、3号药组,每组20只,3个给药组给予相应的中药配方颗粒灌胃,10 d后,除正常组外,其余各组模拟快速上浮脱险诱导减压病模型.观察各组大鼠生存率、肺组织病理、血清和肺组织IL-1、TNF-α的变化情况.结果 与模型组比较,3个给药组大鼠死亡率下降,2、3号药组肺泡壁增厚程度和肺间质充血水肿程度明显减轻.2号和3号药组血清中IL-1、TNF-α含量[（145.84 ±2.10） ng/L,（249.71 ±2.89） ng/L;（123.71 ±1.74） ng/L,（229.23 ±8.74） ng/L]与对照组[（183.18±2.41） ng/L,（300.33±6.03） ng/L]比较均显著下降,差异有统计学意义（P＜0.01）;组织匀浆中IL-1与TNF-α含量亦明显下降（P＜0.01或P＜0.05）.结论 2、3号药组对快速上浮脱险减压病大鼠肺组织的炎症反应有明显的改善作用.本研究为2、3号药组用于减压病的治疗提供了实验依据支撑.

Objective To observe the effects of 3 Chinese medicinal drug formulas on the lung inflammatory response in the rats with decompression sickness induced by fast buoyancy ascent escape, and also to screen out the drug formula that had confirmed curative efficacy.Methods The experimental rats were randomly classified into 5 groups： the normal group, the model group, the No.1 drug group, the No.2 drug group and the No.3 drug group, each consisting of 20 animals.The 3 drug treatment groups were given Chinese medicinal drug granules by gavage.After 10 days, all the animal groups except the normal group underwent simulated fast buoyancy ascent escape to develop the decompression sickness model.Then, close observation was made on the survival rate, lung tissue pathology, and changes in the levels of IL-1and TNF-α in the animals of various groups.Results As compared with that of the model group, survival rates of the rats in the 3 drug treatment groups were increased.Thickening of the alveolar wall and pulmonary interstitial edema were obviously alleviated in the rats of the No.2 and No.3 groups, and the levels of IL-1 and TNF-oα in serum and tissue homogenate were all obviously decreased.Conclusions The No.2 and No.3 drug formals could obviously alleviate lung inflammatory response of the rats with DSC induced by the fast buoyancy ascent escape.This study provided experimental evidence for the application of No.2 and No.3 drug formulas in the treatment of DCS.