摘要
目的 探讨卵泡液白细胞介素（IL）-6、肿瘤坏死因子（TNF）-α的变化对卵巢子宫内膜异位症（简称卵巢内异症）患者体外受精-胚胎移植（IVF-ET）临床结局的影响.方法 收集2013年6月至2014年6月唐山市妇幼保健院64例卵巢内异症患者资料,进行回顾性分析,以男方因素不孕患者58例为对照组,分析比较获卵率、成熟卵（metaphaseⅡ,MⅡ）卵率、受精率、补救-卵胞质内单精子注射（R-ICSI）率、优胚率、生化妊娠率、临床妊娠率,检测卵泡液IL-6、TNF-α的表达变化.结果 卵巢内异症组和男性因素组获卵率（％）分别为73 ±9、92 ±7,MⅡ卵率（％）分别为69 ±8、88 ±5,受精率（％）分别为73±3、87±8,优胚率（％）分别为10±3、31±7,生化妊娠率（％）分别为35 ±4、57 ±4,临床妊娠率（％）分别为23 ±5、55 ±4,两组比较均P＜0.05;卵巢内异症组较男方因素组的R-ICSI率高（10.3％±2.2％;3.5％±1.3％）,P ＜0.05.卵巢内异症组IL-6表达患侧及对侧表达均较男方因素组增加,差异有统计学意义.卵巢内异症组TNF-α表达患侧及对侧均较男方因素组增加,差异有统计学意义.结论 卵巢内异症患者卵母细胞周围卵泡液的炎症微环境有可能影响IVF结局。
Objective To observe the effect of follicular fluid IL-6,TNF-α on the clinical outcome of in vitro fertilization and embryo transfer （IVF-ET） in patients with ovarian endometriosis.Method From June 2013 to June 2014,the data of 64 （from Tangshan Maternal and Child Health Hospital IVF center） ovarian endometriosis patients was analyzed retrospectively.58 infertility cases caused by male side were used as control group.Oocyte retrieval rate,M Ⅱ oocytes rate,fertilization rate,recovery-intracytoplasmic sperm injection （R-ICSI） rate,good quality embryo rate,biochemical pregnancy rate and clinical pregnancy rate were analyzed and compared between two groups.Changes in the expression of follicular fluid IL-6,TNF-α were detected.Results Oocyte retrieval rate,M Ⅱ oocytes rate,fertilization rate,good quality embryo rate,biochemical pregnancy rate and clinical pregnancy rate in ovarian endometriosis group were significantly lower than those in the control group （all P 〈 0.05）,while R-ICSI rate increased in ovarian endometriosis group compared with control group （P 〈 0.05）.IL-6,TNF-α expressions of follicular fluid were higher in affected side of ovarian endometriosis patients than those in the unaffected side and those in control group.Conclusion Inflammation microenvironment of the follicular fluid may influence IVF-ET outcomes in ovarian endometriosis patients.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2015年第47期3829-3832,共4页
National Medical Journal of China
