海洋源放线菌Streptomyces sp.SCSIO 01681次级代谢产物的研究

Studies on the secondary metabolites of marine-derived Streptomyces sp.SCSIO 01681

目的对从中国三亚鹿回头海水沉积物中分离得到的1株海洋放线菌SCSIO 01681进行鉴定并对其次级代谢产物进行研究。方法通过16SrDNA序列分析并构建系统发育进化树来鉴定菌株,利用有机溶剂萃取、正相和反相硅胶层析等分离手段对海洋放线菌SCSIO 01681的发酵产物进行分离纯化,通过波谱数据分析及文献比较对化合物进行结构鉴定,并进行了卤虫致死及抗菌活性评价。结果该放线菌被鉴定为Streptomyces sp.SCSIO 01681,并从其发酵产物中分离得到3个化合物,其结构分别鉴定为苯乙酸(1),亚油酸甘油酯(2),邻苯二甲酸二(2-乙基己基)酯(3);活性结果显示化合物1对嗜水气单胞菌、耐甲氧西林金黄色葡萄球菌、藤黄微球菌、粪链球菌具有抑制作用。结论放线菌Streptomyces sp.SCSIO 01681能够产生3个医药、工业重要中间体,具有潜在的应用价值。

Objective To identify and investigate the bioactive secondary metabolites from the marine-derived SCSIO 01681 isolated from a sediment sample.Methods The strain was classified on the basis of phylogenetic tree analysis of its 16 SrDNA sequence.The fermentation extract was separated by solvent extraction,normal and reverse phase silica gel chromatography.The structures of the purified compounds were elucidated on the basis of spectroscopic data and comparisons with those reported in literatures.The compounds were evaluated for antibacterial and Artemia salina-lethal activities.Results The strain was identified as Streptomyces sp.SCSIO 01681 and three compounds were isolated.Their structures were identified as phenylacetic acid（1）,linoleic acid glyceride（2）and bis（2-ethylhexyl）phthalate（3）.Conclusion We discovered a new marine-derived actinomycete Streptomyces sp.SCSIO 01681 capable of producing three industrial material intermediates with potential application value.