正交试验法优选金银花中绿原酸的提取工艺

Optimization to the Extraction Process for the Cchlorogenic Acid of Lonicera Japonica Thunb by Orthogonal Design

目的:优选金银花中绿原酸的提取工艺。方法:采用正交试验,以紫外分光光度法测定提取物中绿原酸的含量。结果:对绿原酸提取结果影响因素的大小排序为:加水量>浸泡时间>浸泡温度>提取时间,筛选的最佳提取工艺为:浸泡温度为60℃,浸泡时间为0.5 h,加水量20+18倍,提取时间1+0.5 h。结论:优选的提取工艺经济、简便,绿原酸提取效率高。

Objective： To improve the extraction process of the chlorogenic acid of Lonicera japonica Thunb. Methods： The contents of the chtorogenic acid in extract were determined by orthogonal design and UV spectrophotometry measurements. Results： The factor impact on the results sort of the magnitude of the water addition〉soaking time〉 soaking temperature〉extraction time, The optimum 60 ℃ soaking temperature,soaking 0.5 hour, 20＋ 18 times water, 1＋0.5 h extraction. Conclusion： The method was cheap and simple and effective.