功劳木中总生物碱的含量测定

Content Determination of Total Alkaloids in Caulis Mahonia

目的：采用柱色谱-紫外分光光度法测定功劳木中总生物碱（以盐酸小檗碱计）含量的方法,为功劳木药材的质量控制提供科学的依据.方法：用柱色谱-紫外分光光度法,以盐酸小檗碱为对照品,采用盐酸-甲醇（1：100）冷浸后超声提取,经过中性氧化铝柱分离纯化,在265 nm处测定功劳木药材的含量.结果：盐酸小檗碱浓度在2.11～12.66 μg＆#183;mL-1时具有良好的线性关系（r2=0.9995）,平均回收率为98.61％,重复性试验RSD为1.31％（n=6）.结论：柱色谱-紫外分光光度法测定功劳木中总生物碱含量,方法操作简便可靠,重现性好,可以作为功劳木药材的质量控制方法之一.

Objective： To establish column chromatography-UV spectrophotometry for determination of total alkaloids （berberine hydrochloride） in Caulis Mahonia, to provide a scientific basis ibr the quality control of Radix Caulis Mahonia. Methods： The sample was extracted with HCl-CH3OH（1 ： 100） by cold after ultrasonic extraction and total alkaloids were separated on alumina column. Using berberine hydrochloride as control, the content of total alkaloids were determined by UV-spectrophotometry at detection wavelength of 265 nm. Results： The calibration curves fbr berberine hydrochloride was linear in the range of 2.11-12.66 μL.mL-1 （r=0.9995）. The average recovery was 98.61%, and the RSD was 1.31% （n=6）. Conclusion： The method is rapid, accurate and reliable, which can be used for the quality control of Caulis Mahonia.