五华鸡J亚群禽白血病的初步净化

Preliminary Eradication for Avian leukosis Virus- J Subgroup in Wuhua Chicken

禽白血病是由白血病/肉瘤病毒群引起的禽类多种肿瘤型疾病的总称。该病目前尚未有有效的商品化疫苗,控制禽白血病最主要手段是病原体的净化。试验选用繁昌某五华种鸡场父母代鸡群为研究对象,前后5次采集血样共3059份,子代1日龄雏鸡肛门棉拭子采集样本1262份。ELISA法对该群体进行了J-亚群禽白血病（Avian leukosis virus-J subgroup,ALV-J）的抗原、抗体筛查,ALV p27抗原检测试剂盒检测了子代1日龄胎粪中ALV p27含量。分析ELISA检测ALV-J阳性率表明,种鸡ALV-J阳性率较高,达40%;五华鸡保种场的子代雏鸡第1~4批ALV-J阳性率分别为9.09%、5.53%、1.42%、1.30%。由此可见,该场五华鸡父母代鸡群经过1个世代,4批次子代的净化,取得较为理想的效果。分析结果可为后期J亚群禽白血病的持续净化提供基础数据。

Avian leukosis virus（ALV） is the most common oncogenetic retrovirus that emerges spontaneously as a result of recombination between exogenous viruses, exogenous viruses and endogenous viruses, and exogenous viruses and non-homologous cellular genes. While there are no effective commercialized vaccines, pathogen eradication might be more operational. Parental group from Fanchang Wuhua breed conservation station were used for pathogen eradication, 5 batches with a total of 3059 blood samples were collected during July 2012 to April 2013. In addition, 1262 anal swab samples from1 day old chicks were also collected. Antigen and antibody of ALV-J from blood samples were screened using ELISA method.Anal swab samples were also detected using ALV p27 antigen detection kit. The results showed that positive ALV-J in parental group was high to 40%. ALV-J positive rate to the four batches was 9.09%、5.53%、1.42% and 1.30%, respectively.In conclusion, the ALV-J positive rate was controlled in a very low percentage in parental group after this eradication process.These results could be meaningful for the further eradication of ALV-J.