大孔树脂对败酱草多酚的分离及纯化研究

Separation and purification of polyphenols from patrinia by macroporous adsorbent resin

败酱草中多酚含量丰富,在医药领域显示出巨大的应用价值。通过静态吸附法对D3520、H103、NKA、D4006、X-5、D4020、AB-8、S-8、NKA-9和NKA-II10种大孔树脂进行筛选,选出H103型树脂为吸附败酱草中多酚效果最佳的树脂,并研究了其对败酱草中多酚的静态与动态的吸附和解吸性能。结果表明,H103树脂对败酱草多酚的静态饱和吸附量为14.42mg·g^(-1)(干树脂),达到吸附饱和的时间为6h。测定了25、30、35℃下的等温吸附线,且采用Langmuir模型的拟合效果略优于Freundlich模型,为单分子层吸附。此外,样品液浓度、温度、pH值、乙醇浓度等因素均对H103树脂吸附分离败酱草多酚有影响。较理想的工艺参数为:吸附温度30℃,样品液浓度1.34mg·mL^(-1)左右,pH值保持败酱草提取液本身的pH=5.95,动态吸附的上样体积170mL;采用75mL60%乙醇溶液进行动态洗脱。

The content of patrinia polyphenol is rich, which shows great application value in the field of medicine. 10 kinds of macro- porous resins including D3520, H103, NKA, D4006, X-5, D4020, AB-8, S-8, NKA-9 and NKA-II were screened, H103 resin were chosen as the best adsorbing material for the adsorption of patrinia polyphenols by static method, and its performances in adsorbing and desorbing of polyphenols of patrinia was investigated. The results showed that the saturated adsorption capacity of H103 resin was 14.42mg.g-l（dry resin）in static method. The adsorption isotherm data of H103 resin was found that the fitting effect of the Langmuir model was slightly better than the Freundlich model at different temperature of 25℃ ,30℃, 35℃ ,respactively, and it was monolayer adsorption. Furthermore, sample concentration, temperature, pH, ethanol concentration had great effect on macroporous resin in the process of adsorbing and separating patrinia polyphenols. The optimal technological parameters were the temperature of 30℃ ,the sample concentration of 1.34mg. mL-1 , pH 5.95, sample volume of dynamic absorption of 170mL, and 60% ethanol of 75mL as dynamic elution solvent.