miR-210对大鼠蛛网膜下腔出血后迟发型血管痉挛的防治作用

Experimental Study of miR-210 on Effect and Mechanism of Delayed Cerebral Vasospasm after Subarachnoid Hemorrhage of Rats

【目的】探讨mi R-210对蛛网膜下腔出血后迟发性脑血管痉挛(DCVS)的防治作用及其相关机制。【方法】取60只清洁级的成年SD大鼠,雌雄不限,随机分3个实验组:SAH组、对照组、mi R-210治疗组,各组的大鼠数量为20只。SAH组:使用枕大池二次注血方案,制作动物模型;对照组:也采用枕大池注射方案,注射内容为生理性盐水;mi R-210治疗组:SAH模型建立后,mi R-210枕大池注射。SAH模型制备成功后第7天取材测量大鼠基底动脉的管径、壁厚,HE染色观察大鼠基底动脉痉挛的情况及病理变化。应用Western blot技术检测基底动脉TLR4和TNF-α的表达。【结果】1实验结果显示SAH组较对照组相比基底动脉管径明显减小,壁厚明显增大(P<0.01);2mi R-210治疗组与SAH组比较基底动脉管径增大、壁厚减小(P<0.01);3Western blot检测的结果表明,同SAH组比较,TLR4、TNF-α的表达水平在mi R-210治疗组中显著降低(P<0.01)。【结论】1mi R-210缓解SAH后CVS的作用,可能是通过降低TLR-4、TNF-α在基地动脉中的表达而实现的。2蛛网膜下腔出血后迟发性脑血管痉挛的产生与TLR4信号传导通路有一定的关系。

【Objective】 To observe the changes of the diameter of the basilar artery, the thickness of arterial wall, expression of TLR4 and TNF-α after subarachnoid hemorrhag e in the model of SAH of rats, and to explore the preventive treatment and related signaling pathways of mi R-210 in vascular vasospasm after delayed subarachnoid hemorrhage. 【Methods】 Sixty adult healthy SD rats were randomized into 3 groups, 1SAH group 2control group 3miR-210 group. Each had 20 rats. For SAH group, we injected autologous blood into the rats＇ cisterna magna to establish model. For control group, we injected saline instead. For miR-210 group, we injected mi R-210 after the establishment of SAH model. Seven days after injection, the basilar artery spasm was observed with HE staining and the basilar artery diameter, arterial wall thickness were examined. Western blot was used to examine basilar artery ＇s expression of TLR4 and TNF-α. 【Results】1The diameter of basilar artery were significantly smaller in the model group when compared with normal group and the thickness of arterial wall significantly increased（P〈0.01）. 2Compared with SAH group, the basilar artery diameter in mi R-210 group increased and the thickness of arterial wall decreased（P〈0.01）. 3When compared with model group,both TLR4 and TNF-α＇s expression were significantly decreased significantly in the mi R-210 group（P〈0.01）. 【Conclusions】 1mi R-210 could reduce the expression of TLR-4,TNF-α in basilar artery, thus alleviating delayed cerebral vasospasm following subarachnoid hemorrhage. 2The pathway of Toll-like receptor might be of relationship withdelayed cerebral vasospasm（DCVS）.