辅酶Q_(10)对大鼠主动脉粥样硬化斑块的影响

Effect of coenzyme Q_(10) on Wistar rats with aortic atherosclerotic plaques

目的探讨辅酶Q_(10)对Wistar大鼠主动脉粥样硬化斑块病变程度和斑块内胶原含量的影响。方法 38只Wistar大鼠随机选7只为对照组,给予普通饲料;余31只大鼠给予高脂饲料+维生素D3腹腔注射+卵清白蛋白腹腔注射制备动脉粥样硬化模型,13周后随机选取3只大鼠行主动脉组织病理检查HE染色,动脉粥样硬化斑块形成为造模成功。造模成功后将余28只大鼠随机分为动脉粥样硬化模型组(模型组)、辅酶Q_(10)治疗组(辅酶Q_(10)组)、阿托伐他汀治疗组(阿托伐他汀组)、辅酶Q_(10)+阿托伐他汀治疗组(联合组),每组各7只。对照组和模型组给予等体积生理盐水,其余3组分别给予辅酶Q_(10)30mg/(kg·d),阿托伐他汀5mg/(kg·d),辅酶Q_(10)30mg/(kg·d)+阿托伐他汀5mg/(kg·d),连续8周。取主动脉弓行HE染色和Masson染色,运用计算机图像分析仪检测各组斑块面积、胶原面积,血管横截面积,计算校正斑块面积(斑块面积/血管横截面积)与校正胶原面积(胶原面积/血管横截面积)。结果联合组主动脉弓校正斑块面积为(0.04±0.01),低于阿托伐他汀组(0.10±0.02)、辅酶Q_(10)组(0.09±0.02)和模型组(0.17±0.05)(P<0.05),辅酶Q_(10)组和阿托伐他汀组低于模型组(P<0.05),辅酶Q_(10)组与阿托伐他汀组比较差异无统计学意义(P>0.05);联合组主动脉弓校正胶原面积为(0.33±0.03),高于阿托伐他汀组(0.27±0.03)、辅酶Q_(10)组(0.29±0.04)和模型组(0.18±0.03)(P<0.05),辅酶Q_(10)组和阿托伐他汀组高于模型组(P<0.05),辅酶Q_(10)组与阿托伐他汀组比较差异无统计学意义(P>0.05)。结论辅酶Q_(10)可减轻动脉粥样硬化病变程度,增加斑块内胶原含量而增强斑块稳定性。

Objective To explore the effect of coenzyme Q10 on the aortic atherosclerotic plaque lesion degree and collagen content of the plaques in Wistar rats. Methods In 38 Wistar rats, 7 rats were randomly selected as control group receiving normal diet, and the other 31 rats were fed with high-fat diet and intraperitoneally injected vitamin D3 ＋ intraperitoneally injected Ovalbumin to establish atherosclerosis （AS） rat models, which was proved to be successful with HE results of random 3 out of these 31 rats after 13 weeks. Then the left 28 AS model rats were randomly divided into model group, coenzyme Q10 group, atorvastatin group and combination group, with 7 rats in each group. Control group and model group were given normal saline, coenzyme Q10 group was given coenzyme Q10 30 mg/（kg · d）, atorvastatin group was given atorvastatin 5 mg/（kg· d）, and combination group was given coenzyme Q10 30 mg/（kg · d） ＋ atorvastatin 5 mg/（kg · d） for 8 weeks. The aortic arch processed by HE and Masson staining. Computer image analyzer was used to measure plaque area, collagen area and vascular cross-sectional area, and to calculate the corrected plaque area （plaque area/vascular cross-sectional area） and corrected collagen area （collagen area/vascular cross-sectional area）. Results The corrected plaque area was lower in combination group （0.04±0.01） than that in atorvastatin group （0.10± 0.02）, coenzyme Q10 group （0.09±0.02） and model group （0.17±0.05） （P〈0.05）, was lower in coenzyme Q10 group and atorvastatin group than that in model group （P〈0.05）, and there was no significant difference between coenzyme Q10 group and atorvastatingroup （P2〉0.05）. The corrected collagen area was higher in combination group （0.33±0.03） than that in atorvastatin group （0. 27±0. 03）, coenzyme Q10 group （0.29±0.04） and model group （0. 18±0.03） （P〈 0.05）, higher in coenzyme Q10 group and atorvastatin group than that in model group （P〈0.05）, and there was no significant difference between coenzyme Q10 group and atorvastatin group （P〉0.05）. Conclusion Coenzyme Ql0 can reduce the AS degree, as well as increase the plaque collagen content and plaque stability.