辐射白刺花遗传多样性的ISSR和RAPD比较分析

Comparison of Genetic Diversity of Irradiated Sophora vicii folia Using ISSR and RAPD Markers

采用ISSR和RAPD标记对8个^(60)Co-γ辐射梯度诱变白刺花(Sophora viciifolia)进行遗传多样性分析。结果表明:20条ISSR和6条RAPD引物的PPL多态率分别为51.37%和21.03%,ISSR的Nei's指数和平均Shannon指数均大于RAPD;ISSR的标记指数(1.28)是RAPD(0.20)的6.4倍,表明ISSR具有较高的标记效率。Mantel检测表明,ISSR+RAPD标记与ISSR标记的结果显著相关(P<0.05)。综合分析表明白刺花用1SSR标记可检测出更高的多态性,更能准确反映白刺花材料间的遗传关系。

The RAPA and ISSR markers were used in analyzing the genetic diversity of mutagenic Sophora vicii folia Franch with eight different ∽（60）Co-γ-rays gradients.The results showed that the percentage of polymorphic bands of 20 ISSR primers and 6 RAPD primers were 51.27%and 21.03%,respectively.The Nei＇s gene heterozygosity and Shannon＇s information index of ISSR were greater than RAPD.For 8 materials,ISSRs got a MI value（1.28） that was 6.4 times higher than that of RAPDs（0.20）,indicating that ISSRs had a higher MI efficiency.The Mantel test revealed that the correlation was significant between ISSR＋RAPDs and ISSRs（r=0.9791,P0.05）.Comprehensive research showed that ISSRs could detect the higher polymorphism of Sophora vicii folia,and more accurately reflect the genetic relationship among Sophora vicii folia.