摘要
为提高兼并引物PCR的特异性和扩增效率,改进了兼并引物的设计,在兼并引物上加入接头序列,使其延长,并利用该方法成功获得了一个新的聚酮类合成酶的基因片段,表明抑制性PCR能够提高兼并引物扩增的效率及特异性.
To enhance the specificity and efficiency of degenerate primer PCR,the design of degenerate primer was improved,adaptor sequences were added to the degenerate primer to prolong it.And a DNA fragment of a novel polyketide synthase gene was obtained by this method,which indicates that suppression PCR can be used to enhance the amplification efficiency and specificity of the degenerate primer.
出处
《杭州师范大学学报(自然科学版)》
CAS
2015年第6期581-584,共4页
Journal of Hangzhou Normal University(Natural Science Edition)
基金
国家自然科学基金青年项目(31101642
31201090)
关键词
抑制性PCR
兼并引物
分子克隆
PCR suppression effect
degenerate primer
molecular cloning