妊娠高血压患者血管内皮生长因子及胎盘生长因子含量的变化及其意义

Changes and significance of serum levels of vascular endothelial growth factor and placental growth factor in patients with hypertensive disorder complicating pregnancy

目的:观察血管内皮生长因子(VEGF)与胎盘生长因子(PLGF)在孕早、中期的妊娠期高血压(HDCP)患者的表达,为诊断妊娠期高血压提供依据。方法:选择46例正常妊娠(正常妊娠组)和39例HDCP(HDCP组)患者作为研究对象,HDCP组又分为:妊娠高血压期(26例)、轻度子痫前期(8例)和重度子痫前期(5例),采用酶联免疫吸附法(ELISA)检测各组早、中、晚期血清VEGF与PLGF水平,并进行比较。结果:与正常妊娠组比较,HDCP组孕中期(24周)血清VEGF[(187.54±17.36)ng/L比(123.35±16.46)ng/L]与PLGF[(217.49±26.32)ng/L比(128.32±21.23)ng/L]水平明显降低(P均<0.01),且孕晚期(34周)血清VEGF和PLGF水平下降更显著(P<0.01);在HDCP组中,孕24周时,与妊娠期高血压和重度子痫前期比较,轻度子痫前期PLGF[(127.46±16.55)ng/L、(158.40±26.33)ng/L比(99.02±27.26)ng/L]水平明显下降;且34周时VEGF和PLGF水平下降更显著(P<0.05或<0.01)。结论:妊娠高血压患者孕中期血清VEGF与PLGF水平降低有助于妊娠高血压的早期诊断。

Objective： To observe expressions of vascular endothelial growth factor （VEGF） and placental growth factor （PLGF） in patients with hypertensive disorder complicating pregnancy （HDCP） during early and mid pregnancy, and pro- vide basis for diagnosing HDCP. Methods： A total of 46 normal pregnant women （normal pregnancy group） and 39 HDCP patients （HDCP group） were enrolled. The HDCP group was further divided into gestational hypertension group （n = 26）, mild preeclampsia group （n = 8） and severe preeclampsia group （n = 5）. Enzyme linked immunosorbent assay （ELISA） was used to measure serum VEGF and PLGF levels during early, mid and late pregnancy, and those were compared among a- bove groups. Results： Compared with normal pregnancy group, there were significant reductions in serum levels of VEGF [ （187. 54±17. 36） ng/L vs. （123.35±16.46） ng/L] and PLGF [ （217. 49±26. 32） ng/L vs. （128. 32±21.23） ng/L] in HDCP group during mid period （24th week）, P〈0.01 all, and serum VEGF and PLGF levels more significantly reduced during late pregnancy （34th week） （P〈0.01）; in HDCP group, compared with gestational hypertension group and severe preeclampsia group, PLGF level [ （127. 46 ± 16. 55） ng/L, （158. 40± 26. 33） ng/L vs. （99.02±27.26） ng/L] significantly reduced in mild preeclampsia group on 24th week; the serum VEGF and PLGF levels more significantly reduced on 34th week, P〈0. 05 or 〈0.01. Conclusion： Reduced serum levels of VEGF and PLGF during mid pregnancy in HDCP patients are help to early diagnosing HDCP.