白芦藜醇对自发性高血压大鼠血管重构的保护作用

The protective effect of resveratrol on arterial remodeling in spontaneous hypertensive rats

目的观察白芦藜醇对自发性高血压大鼠血管重构的作用。方法选择20只雄性自发性高血压大鼠（SHR）和10只wistar-Kyoto（wKY）大鼠，数字抽签随机分为对照组、模型组及白芦藜醇组。观察血压、脉搏波速度变化情况，采用苏木-伊红（HE）染色、维多利亚蓝天狼星红染色观察主动脉形态学改变，检测一氧化氮（NO）、内皮素1（ET1）观察血管内皮功能，检测超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、过氧化氢（H202）观察氧化应激水平。结果22周末，与对照组比较，模型组大鼠出现血压升高，脉搏波速度增加，主动脉管壁增厚，壁厚内径百分比增大，血管横截面积及血管腔横截面积增大，胶原纤维面积／弹性纤维面积比值增加。主动脉组织中NO、SOD、CAT表达下降而ET-1、MDA、H2O2表达增加。经白芦藜醇处理后，与模型组比较，白芦藜醇组血压无明显变化，而脉搏波速度下降（6681．1±2154．9）cm／s与（4283．1±946．1）cm／s（P〈0．05），主动脉管壁厚度降低（P〈0．01），壁厚内径百分比减小（P〉0．05），血管横截面积减小（P〈0．05），血管腔横截面积减小（P〈0．05）；胶原纤维面积／弹性纤维面积比值减小（P〈0．01）；主动脉组织中NO表达增多（P〈0．01），SOD表达增多（P〈0．01），CAT表达增多（P〈0．05），ET-1表达减少（P〈0．01），MDA表达减少（P〈0．01），H202表达减少（P〈0．05）。结论白芦藜醇具有抗氧化作用，可通过改善氧化应激，延缓血管重构的过程。

Objective To observe the effect of resveratrol on arterial remodeling in spontaneous hypertensive rats. Methods Spontaneous hypertensive rats （SHRs, n=20） and Wistar-Kyoto rats （WKYs, n = 10 ） were randomly assigned to three groups： control group, model group and resveratrol group. Systolic blood pressure （SBP） and pulse wave velocity （PWV） of the three groups was observed. Morphological observations were obtained by hematoxylin and eosin or victoria blue and picrosirius red. The content of nitric oxide （NO） and endothelin-1 （ET-1） were determined to assess the endothelial function. Oxidative stress was assessed by malondialdehyde （MDA）, hydrogen peroxide （H2 O2） , and activities of antioxidant enzymes superoxide dismutase （SOD） and catalase （CAT）. Results Compared with control group, SBP, PWV, wall thickness, wall-lumen ratio, vascular cross-sectional area （VCSA）, luminal cross-sectional area （LCSA） and collagen-elastic ratio were increased, and the levels of NO, SOD,CAT in rat aortic tissues were decreased, while ET1, MDA, H202 levels in rat aortic tissues were enhanced in model group at the end of 22 weeks. After the treatment of resveratrol, SBP had no significantly difference between model and resveratrol groups, while PWV, aortic wall thickness, wall-lumen ratio, VCSA, LCSA and collagen-elastic ratio were decreased in resveratrol group as compared with model group [（6681.1±2154.9） cm/s w （4283.1±946.1） cm/s, （234.7±51.8）μm vs. （123.4±21.5）μm, （10.3±2.3）% vs. （5.8±1.4）%, （6.4±0.4）mm^2 w （4.8±0.6）mm^2, （4.1±0.1）mm2 vs （3.2±0.4）mm^2, （1.1±0.3） w （0.4±0.2）, P〈0.05 or 0. 013. Compared with model group, the expressions of NO, SOD, CAT in rat aortic tissues were increased and levels of ET-1, MDA, H2O2 in rat aortic tissues were decreased in resveratrol group after treatment of resveratrol （P〈0.01 or P〈0.05）. Conclusions Resveratrol has an antioxidant effect, and it could attenuate arterial remodeling by improving oxidative stress.