金银忍冬花粉生活力测定方法的研究

Study on Determination Method of Pollen Viability of Lonicera maackii(Rupr.)Maxim

为了探索金银忍冬花粉萌发活力的快速检测方法,以金银忍冬花粉为试材,采用醋酸洋红染色法、TTC染色法和过氧化物酶染色法3种不同染色方法及离体萌发培养法对金银忍冬花粉生活力进行测定。结果表明:最适宜的离体萌发培养基为15%蔗糖+160mg·L-1硼酸;过氧化物酶染色法的测定值与离体萌发培养法的测定结果最相近,是测定金银忍冬花粉生活力的最适染色法。

Pollen of Lonicera maackii（Rupr.）Maxim.was employed as the experimental material,determination method of Lonicera maackii（Rupr.）Maxim.＇s pollen viability was studied.Comparisons were made for the effect of sucrose and boron in culture solution on the germination of Lonicera maackii（Rupr.）Maxim.＇s pollen.And carmine acetate staining,TTC staining and peroxidase staining were compared in order to seek for a fast determination method of Lonicera maackii（Rupr.）Maxim.＇s pollen viability.The results indicated that the optimum culture solution was 15%sucrose＋160mg·L-1 H3BO3;and the result of peroxidase staining was similar to that of germination,it was the most optimal staining method for testing pollen viability of Lonicera maackii（Rupr.）Maxim.