超高效液相色谱-串联质谱法测定人血浆中氯苯那敏

Determination of Chlortrimeton in Human Plasma with Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

研究并建立了测定人血浆中氯苯那敏的超高效液相色谱-串联质谱（UPLCMS/MS）分析方法。血浆样品中加入内标,经2.5倍体积甲醇沉淀蛋白,涡旋离心后,取上清液进样测定。采用Waters ACQUITY UPLC HSS C18色谱柱（150×2.1mm,1.8μm）分离,以5mmol/L乙酸铵水溶液（含0.1%甲酸）-甲醇为流动相,梯度洗脱,流速为0.2 mL/min。氯苯那敏的线性范围为0.10~50.0μg/L,相关系数（r2）为0.9995,检出限和定量限分别为0.05、0.10μg/L。该方法操作简便、专属性强、灵敏度高,适用于人体血浆中氯苯那敏的测定。

A method for detection of Chlortrimeton in human plasma has been developed using ultra performance liquid chromatography combined with tandem mass spectrometry（UPLC-MS/MS）.After added the internal standard,human plasma samples were precipitated to remove proteins with 2.5times volume of methanol and vortex-centrifuged.The supernatants were injected into the UPLC-MS/MS system.The separation was performed on a Waters ACQUITY UPLC HSS C18column（150×2.1mm,1.8μm）at 25℃,using methanol and 0.1%formic acid with 5mmol/L ammonium acetate in water as the mobile phase with the flow rate at 0.2mL/min.The linear range for the detection of Chlortrimeton in human plasma was 0.10-50μg/L with the correlation coefficient of 0.9995.The limit of detection was0.05μg/L and the limit of quantitation was 0.10μg/L.The method is easy to operate,strongly selective,and highly sensitive for detection of Chlortrimeton in human plasma.