败毒散中5种有效成分的HPLC法测定

Determination of Five Effective Components in Baidu San by HPLC

建立了高效液相色谱法测定败毒散中的5种有效成分,即蛇床子素、二氢欧山芹醇当归酸酯、洋川芎内酯I、洋川芎内酯A和欧当归内酯A。使用C18色谱柱,以乙腈∶0.1%冰乙酸为流动相,梯度洗脱,检测波长为330 nm（0~28 min）和280 nm（28~60 min）。上述5种成分分别在5.0~100.0、2.6~52.0、2.2~44.0、8.5~170.0和2.0~40.0μg/ml范围内线性关系良好。平均回收率分别为98.25%、96.84%、99.06%、99.22%和97.74%,RSD分别为1.27%、0.81%、1.22%、1.05%和1.43%。

An HPLC method was established for the determination of five effective components, osthole, columbianadin, senkyunolide I, senkyunolide A and levistilide A, in Baidu San. A C18 column was used, with the mobile phase of acetonitrile ; 0.1% glacial acetic acid by gradient elution, at the detection wavelengths of 330 mn （0 - 28 min） and 280 mn（28 - 60 Min）. It was linear for the above five compounds in the ranges of 5.0 - 100.0, 2.6 - 52.0, 2.2 - 44.0, 8.5 - 170.0, and 2.0 - 40.0 μg/ml, respectively. Their average recoveries were 98.25%, 96.84%, 99.06%, 99.22% and 97.74 %, with RSDs of 1.27 %, 0.81%, 1.22 %, 1.05 % and 1.43 %, respectively.