Au微球阵列跨尺度葡萄糖传感器的制备

Construction of Glucose Sensors Based on the Arrays of Au Microspheres

以硅基底和光纤纤芯阵列为定位夹具,手工排列熔凝法得到的Au微球,获得3×3 Au微球阵列;在Au微球表面先用水浴法合成ZnO纳米线、后固定葡萄糖氧化酶,得到球形跨尺度阵列葡萄糖酶电极。对球形跨尺度结构的表面形貌、葡萄糖氧化酶的固定效果、酶电极的电化学性能进行了定量表征。球形跨尺度结构阵列葡萄糖传感器的线性范围为0~2.5mmol/L、灵敏度为17.24μA·(mmol/L)^(-1)·cm^(-2)、最低检出限为3.44μmol/L、Michaelis-Menten常数为1.85 mmol/L。

With silicon substrates and arrays of optical fiber cores as assembly fixtures,3×3 arrays of Au microspheres produced by melting and condensing Au fibers were manually arranged.ZnO nanowires were hydrothermally synthesized on Au microspheres,and glucose oxidase was immobilized on ZnO nanowires,obtaining the spherically hierarchical structure array-based working electrodes.The surface morphologies and the glucose oxidase immobilization effects of the spherically hierarchical structures as well as the electrochemical performance of the working electrodes were quantitatively characterized.The linear range,sensitivity,low detection limit and Michaelis-Menten constant of this glucose sensor are 02.5 mmol/L,17.24 μA·（mmol/L）-1·cm-2,3.44 μmol/L,and 1.85 mmol/L respectively.