JNK信号途径蛋白P-JNK和P-c-Jun在变应性鼻炎大鼠鼻黏膜组织重塑中的作用

Role of P-JNK and P-c-Jun of JNK transduction pathway on the nasal mucosa remodeling in allergic rhinitis rats

目的:探讨c-Jun氨基末端激酶(JNK)信号途径蛋白P-JNK和P-c-Jun在变应性鼻炎(AR)大鼠鼻黏膜组织重塑过程中的作用。方法:将60只Wistar大鼠以随机数字表法分为AR组(A组)和对照组(B组)。A组以卵清蛋白(OVA)、氢氧化铝建立AR鼻黏膜重塑模型,根据致敏和激发时间不同,分为4、8、12周组(分别为A4、A8、A12组);同时设立相应对照组(分别为B4、B8、B12组),以生理盐水代替OVA。苏木精-伊红染色观察2组大鼠各时期鼻黏膜组织的病理变化;免疫组织化学法检测鼻黏膜组织磷酸化JNK(P-JNK)及磷酸化c-Jun(P-cJun)蛋白的表达。结果:A组大鼠8周时鼻黏膜水肿,黏膜层增厚,并有大量以EOS为主的炎性细胞浸润;随着致敏和激发时间的逐渐延长,上述炎性症状明显加重。A组各时期P-JNK及P-c-Jun(mA值)均高于同期B组(均P<0.01),且12周时二者均高于4、8周时(均P<0.01)。结论:JNK信号途径蛋白P-JNK和P-c-Jun在AR大鼠鼻黏膜重塑组织中表达显著增高,提示P-JNK和P-c-Jun参与了AR大鼠鼻黏膜组织重塑的过程。

Objective：To study the role of P-JNK and P-c-Jun of JNK（c-Jun N-terminal kinase）on nasal mucosa remodeling in allergic rhinitis rats.Method：Sixty male Wistar rats（weighing about 200-250g）were randomly divided into AR group（A group）and B group（control group）.The rats in A group were sensitized for inducing AR by intraperitoneal injection of ovalbumin and Al（OH）3.Rats in group A were randomized into A4,A8 and A12group（each had 10rats）.Ovalbumin was dropped in each nasal cavity of every rat for 4,8,12 weeks,respectively.Rats in group B were sensitized by saline instead of OVA,and were also divided into B4,B8 and B12group.Each group had 10 rats.Pathological changes of nasal mucosa in each period were observed by hematoxylin and eosin stain dyeing.The phosphorylation of JNK and c-Jun were tested by immunohistochemistry.Result：In A8 group,mucosal congestion and edema thickening with inflammatory cells infiltration of eosinophils were observed in the eighth week,and the inflammatory changes were significantly increased as time went on.The mean absorbance values of P-JNK and P-c-Jun in A group were significantly higher than those in the corresponding B group（all P〈0.01）.Moreover,the mean absorbance values of A12 group were significantly higher than A4 group and A8group（all P〈0.01）.Conclusion：The expression of P-JNK and P-c-Jun in the process of nasal mucosa remodeling in allergic rhinitis rats were increased,which suggested that P-JNK and P-c-Jun played important roles in nasal mucosa remodeling of the allergic rhinitis rats.