18β-甘草次酸钠滴鼻对变应性鼻炎大鼠鼻黏膜上皮纤毛的影响

The effect of 18β-sodium glycyrrhetinic acid on the nasal mucosa epithelial cilia in rat models of allergic rhinitis

目的:观察18β-甘草次酸钠滴鼻对变应性鼻炎(AR)大鼠鼻黏膜上皮纤毛的影响。方法:卵清蛋白(OVA)致敏Wistar大鼠建立AR动物模型。造模成功后,大鼠随机分为正常对照组、模型组、布地奈德组(0.2mg/kg剂量)和甘草次酸钠组(20mg/kg剂量和40mg/kg剂量),各组分别在给药2周和4周后比较各组大鼠行为学评分,取鼻中隔黏膜电镜下观察形态学变化。结果:模型组大鼠2周和4周均出现典型的AR症状,行为学叠加评分>5分;经布地奈德和甘草次酸钠干预后,AR症状减轻,行为学叠加评分较模型组均明显降低(P<0.01)。与模型组比较,2周布地奈德组和甘草次酸钠组大鼠鼻黏膜纤毛柱状上皮顶端的稠厚黏液毯状分泌物明显减少,纤毛粘连、倒伏、脱落程度明显减轻,且布地奈德组略好于甘草次酸钠组;4周布地奈德组和甘草次酸钠20mg/kg组纤毛倒伏、脱落均完全消失,且纤毛排列具方向性。甘草次酸钠20mg/kg组较布地奈德组及甘草次酸钠40mg/kg组的纤毛更整齐、光滑,纤毛状态更接近正常对照组。结论:18β-甘草次酸钠可显著抑制AR模型大鼠鼻黏膜纤毛的病理改变。

Objective：To investigate 18β-sodium glycyrrhetinic acid impact on nasal mucosa epithelial cilia in rat models of allergic rhinitis（AR）.Method：AR models were established by ovalbumin-induction.Wister rats were randomly divided into groups as normal group,model group,budesonide（0.2 mg/kg）group and sodium glycyrrhetinic acid（20mg/kg and 40mg/kg）group after the success of AR models.At 2weeks and 4weeks after treatment,the behavioral changes of rats were observed and recorded,and nasal septum mucosae were collected after 2week and 4week intervention,and the morphological changes of nasal mucosae were observed by electron microscope.Result：Model group developed typical AR symptoms,the total score in all animals was 〉5.With budesonide and sodium glycyrrhetinic acid treatment,the AR symptoms were relieved,and the total scores were reduced significantly（P〈0.01）.Compared with the model group：after 2 weeks＇ intervention,thick mucous secretions on the top of columnar epithelium cilia in rat nasal mucosa was significantly reduced,and cilia adhesion,lodging,shedding were relieved in budesonide group and sodium glycyrrhetinic acid group,the relieve in budesonide group was slightly better than that in sodium glycyrrhetinic acid group;after 4week intervention,Cilia adhesion,lodging,shedding were completely vanished,and the cilia were ranged in regular direction in budesonide group and sodium glycyrrhetinic acid group.Cilia in sodium glycyrrhetinic acid（20mg/kg）group was more orderly,smooth than that in budesonide group and sodium glycyrrhetinic acid group（40mg/kg）,and the condition of cilia in sodium glycyrrhetinic acid group（20mg/kg）was similar to the normal group.Conclusion：18β-sodium glycyrrhetinic acid is effective to restrain the pathological changes of nasal mucosa cilia in rat models of AR.