慢性应激对小鼠空间学习记忆的影响及脑内Wnt3a的表达

Effects of chronic stress on ability of spatial learning-memory and expression of Wnt3a in mice brain

目的:研究慢性应激对小鼠空间学习记忆的影响及机制。方法 :慢性应激模型的建立采用的是持续21天的7种不同应激方式,通过Morris水迷宫实验检测小鼠的空间学习记忆能力,免疫组织化学方法检验小鼠脑内各脑区wnt3a的表达。结果:慢性应激组小鼠与对照组小鼠相比,在定位航行试验中,小鼠寻找平台的逃避潜伏期明显提高(P<0.05),空间搜索实验中,应激组小鼠在目标象限的游泳时间明显减少(P<0.05)。在小鼠脑内PFC区wnt3a表达显著减少(P<0.01)。结论 :慢性应激对小鼠空间学习记忆的能力具有损伤作用,这与wnt3a在脑内的表达关系密切。

Objective To study the effects of chronic stress on the spatial learning-memory function of mice and its mechanism. Method The chronic stress model of mice was induced by stimulation of multiple stressors for 21 days. The ability of spatial learning-memory of mice were determined by Morris Water maze task, and the expression of Wnt3a in the hippocampus and prefrontal cortex were detected by immunohistochemical method .Result After chronic stress, the stress group＇s escape latency in the place navigation test were significantly higher than control group mice（P〈 0.05）. In the spatial search test, after chronic stress ,respectively compared with control group mice, in first quadrant （i.e. platform quadrant） the swimming time of stress group mice were obviously shortened （P〈0.05）. TheWnt3a expression in PFC were significantly shortened in stress group mice （P〈0.01）. Conclusion The ability of chronic stress on spatial learning and memory in mice has the function of damage, which is closely related to the expression of Wnt3a in brain.