13种海南入侵植物提取物对降香黄檀炭疽病菌的抑制作用

Inhibitory effects of the extracts of 13 invasive plants from Hainan against Colletotrichum gloeosporioides on Dalbergia odorifera

本文研究了13种海南入侵植物不同溶剂提取物对降香黄檀炭疽病菌的生物活性,以筛选出具有较好抑菌活性的植物提取物。结果表明:10种植物对炭疽病菌有一定的抑菌效果,其中效果最好的提取物为垂序商陆乙酸乙酯提取物,EC50为3.413mg/mL,其次是胜红蓟乙酸乙酯提取物以及飞机草乙醇提取物,EC50分别为5.849、21.984mg/mL。3种植物提取物都可以导致炭疽病菌菌丝畸形,高浓度垂序商陆与胜红蓟提取物甚至可以导致细胞壁破裂。垂序商陆提取物的MIC为5.0mg/mL,MBC为10mg/mL;胜红蓟提取物的MIC为7.5mg/mL,而MBC大于12.5mg/mL。

To screen the plant extracts with high inhibitory activity against Colletotrichum gloeosporioides on Dalbergia odorifera ,the inhibitory activity of the extracts of 13 invasive plants was tested.The results showed that 10 plant extracts had high inhibitory activity against the pathogen.Ethyl acetate extracts of Phytolacca americana had the highest inhibito-ry activity,with an EC50 of 3.413 mg/mL.Ethyl acetate extract of Ageratum conyzoides and ethanol extract of Eupatori-um odoratum also had high inhibitory activity,with an EC50 of 5.849 mg/mL,21.984 mg/mL,respectively.The results of observation under microscope showed that the hyphae of Colletotrichum became misshapen after treated with extracts of the 3 plants,and broken after treated with P .americana and A .conyzoides extracts at high concentration.The MIC and MBC of ethyl acetate extracts of P .americana were 5.0 mg/mL and 10 mg/mL,respectively.The MIC of ethyl ac-etate extract of A .conyzoides was 7.5 mg/mL,and the MBC was greater than 12.5 mg/mL.