甘肃省苹果蠹蛾不同地理种群遗传多样性分析

Genetic diversity analysis of different geographical populations of the codling moth,Cydia pomonella(L.)in Gansu Province

为探明苹果蠹蛾不同地理种群的遗传变异、种群分化及传播扩散情况,利用9对微卫星引物对甘肃9个不同苹果蠹蛾地理种群的遗传多样性进行了研究。应用 POPGENE 1.32和 NTSYSpc 2.1软件分析表明：9对引物共检测到21个多样性位点,多态性百分比为95.45%；9个不同地理种群的观测等位基因为1.9545,有效等位基因为1.7413,Nei 氏指数为0.4113,Shannon 指数为0.5927±0.1472；遗传相似度在0.3258-0.8170范围内。UPG-MA 聚类分析表明甘州、金塔种群聚为一支,与肃州种群合为一支；临泽种群与总寨种群聚为一支；七里河与敦煌种群聚为一支；高台与民勤种群聚为一支,各分支间的遗传分化系数 Fst 分别为0.7950、0.5314、0.8579、0.5980。Mantel 检测表明,种群间的遗传距离与地理距离无显著相关性。研究表明,甘肃苹果蠹蛾种群的遗传多样性较低,遗传变异主要来自种群内部,种群之间的基因交流较少,在甘肃省的传播以人为被动传播扩散为主。

In order to explore the genetic variation,population differentiation and dispersal pattern,nine pairs of primers were used to analyze the genetic diversity of nine different geographical populations of the codling moth, Cydia pomonella （L.）,collected from Gansu.The analysis results with POPGENE 1.32 and NTSYSpc 2.1 pro-grams showed that 21 polymorphic sites were detected from nine different geographical populations,with a poly-morphism percentage,observed number of alleles,effective number of alleles,Nei’s gene identity,Shannon index and genetic similarity of 95.45%,1.954 5,1.741 3,0.41 1 3,0.592 7±0.147 2,and 0.325 8-0.81 7 0,respec-tively.UPGMA cluster analysis indicated that the populations from Ganzhou,Jinta and Suzhou gathered in one clade,the populations from Linze and Zongzhai in another;the populations from Qilihe and Dunhuang clustered in one clade,while the populations from Gaotai and Minqin gathered in another.The genetic differentiation coef-ficient （Fst ）of each clade was 0.795 0,0.531 4,0.857 9 and 0.598 0,respectively.Mantel test indicated that the genetic distance and geographical distance had no significant correlation between different populations.The results showed that codling moth populations had lower genetic diversity,and genetic variation occurred within popula-tion;there was low gene flow between populations,and the mode of diffusion was anthropochory in Gansu Province.