芒果细菌性黑斑病菌在不同场所的存活期

Survival period of Xanthomonas campestris pv.mangiferaeindicae in different places

选择芒果细菌性黑斑病菌（Xanthomonas campestris pv.mangiferaeindicae ,Xcm）抗利福平菌株 RifXcm,通过人工模拟接种试验,采用半选择性平板分离、菌落 PCR 和常规 PCR 方法,研究该病原细菌在芒果叶片、土壤和水中的存活期,并对其能否作为侵染源进行了评价。结果表明,芒果细菌性黑斑病菌在芒果叶片病斑内可存活5～6个月,是此病发生最主要的侵染源。病原菌在土壤和自然水中的存活期有限,其中以含芒果残体土壤中的病原菌存活期最长（49～63 d）,但也没有超过3个月,因此年前存在于这些场所的病原菌均不可能成为第2年的初侵染源。

Mango bacterial black spot,caused by Xanthomonas campestris pv.mangiferaeindicae （Xcm）,is one of the most devastating diseases on mango （Mangifera indica L.）.Survival period of Xcm in different places was preliminary investigated,and whether the pathogen could be the infection source was also evaluated.The survival period of Xcm in mango leaf,soil and water inoculated with anti-rifampicin strain RifXcm was investigated by plate isolation,colony PCR and conventional PCR.The results showed that Xcm could survive in the mango leaf for 5-6 months,which was the major infection source.Survival period of Xcm was very limited in diseased soil and water,of which Xcm in the soil with the diseased mango debris was the longest-living survivors who could only survive for 49-63 d and hardly endured 3 months.Therefore,Xcm in these places couldn’t be the primary infec-tion source.