不同配比头孢哌酮钠/舒巴坦对革兰阴性杆菌体外抗菌效果影响

Effect of Different Proportions of Cefoperazone / sulbactam against Gram-negative Bacteria in Vitro

目的探讨头孢哌酮钠/舒巴坦75/30μg(SCF105)和75/75μg(SCF150)两种配比药敏纸片对革兰阴性杆菌的体外抗菌效果。方法用纸片扩散法检测SCF105和SCF150两种药敏纸片对临床分离的86株革兰阴性杆菌的抑菌环直径,比较药敏结果并分析两种药敏纸片对4种常见革兰阴性杆菌(铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌及鲍曼不动杆菌)的抗菌效果。结果本研究检测86株革兰阴性杆菌的敏感率和中介率SCF150纸片均高于SCF105纸片(P<0.01);SCF150纸片全部菌株的耐药率低于SCF105纸片(P<0.01)。对4种革兰阴性杆菌的敏感率SCF150纸片均高于SCF105纸片,其中差异最明显的是大肠埃希菌(P<0.05)。对全部菌株及4种革兰阴性杆菌抑菌环直径SCF105纸片均小于SCF150纸片(P<0.05)。4种革兰阴性杆菌抑菌环直径两两比较显示,SCF105纸片中大肠埃希菌与2种菌株(铜绿假单胞菌及鲍曼不动杆菌)以及SCF150纸片中大肠埃希菌与其他3种菌株抑菌环直径比较差异均有统计学意义(P<0.05)。结论成分配比为75/75μg的头孢哌酮钠/舒巴坦药敏低纯对革兰阴性杆菌的体外抗菌效果更优。

Objective To explore the differences of antibacterial effect in vitro between two different concentrations of Cefoperazone-Sulbactam（ 2∶ 1 and 1∶ 1） 75 /30 μg（ SCF105） and 75 /75 μg（ SCF150） upon gram-negative bacilli. Methods Susceptibility test of 86 strains of clinical isolated gram-negative bacilli was conducted with K-B method using SCF105 and SCF150 respectively and the diameter of inhibition zone were measured. The Susceptibility results of two kinds of susceptibility sheets were analyzed and the antimicrobial results on the four common gram-negative bacillus（ Pseudomonas aeruginosa,e. Coli,klebsiella pneumoniae and acinetobacter baumannii） was explored. Results For all the 86 strains,susceptibility rate and intermediate rate SCF 150 disc were 53. 5% and 26. 7%,which were significantly higher than that with SCF105 disc; the resistance rate with SCF disc was 19. 7%,significantly lower than the resistance rate of SCF105 disc（ P〈0. 01）. Wilcox on signed rank sum test for the four kinds of clinical common gram negative bacterium（ Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumoniae and Acinetobacter baumannii） was used and sensitivity rate with SCF150 disc was higher than the result of SCF105 disc,and the difference was significant,E. Coli（ P〈0. 05）; For all strains and four gram negative bacteria,the inhibitor diameter of SCF105 disc was smaller than that of SCF150 disc（ P〈0. 05）,and especially in E. coli（ P〈0. 05）. Compared with the inhibitor diameter 4 kinds gram-negative bacilli,there were significant differences between SCF105 of E. Coli and Pseudomonas aeruginosa and acinetobacter baumannii）; meanwhile,there were significant differences between SCF150 of E. coli and 3 other kinds（ P〈0. 05）. Conclusion AS far as antibacterial effectiveness of gram-negative bacilli in vitro is concerned,composition ratio of 75 /75 μg Cefoperazone-Sulbactam is superior to that of 75 /30 μg Cefoperazone-Sulbactam.