3种显色反应对稻米籽粒中原花色素含量的检测分析

Determination and Analysis of the Proanthocyanidins Content in Rice Grain Based on Three Color Reaction

利用二甲基乙酰胺二甲基缩醛(DMACA)染色法、铁铵矾染色法和香草醛染色法,测定了不同品种稻米籽粒中原花色素的含量。用DMACA染色法测得谭农s/制21、R288、凡-14、12庚351、秦稻2号籽粒中原花色素的质量分数依次为0.136,0.183,0.085,4.115,24.736 mg/g;用铁铵矾染色法测得的质量分数依次为0.292,0.094,1.940×10^(-4),1.220,8.080 mg/g;用香草醛染色法测得的质量分数依次为1.349,1.363,1.093,3.733,15.153 mg/g。检测结果表明:5种稻米品种中,秦稻2号的原花色素含量最高,是12庚351的4.1~6.6倍,凡-14中的含量最低;黑米品种(秦稻2号和12庚351)比白米品种(R288、谭农s/制21和凡-14)中原花色素的含量明显要高。吸光度相对标准偏差(RSD)值表明,3种染色法测定原花色素含量的精密度由高到低依次为:香草醛染色法、DMACA染色法、铁铵矾染色法。

The proanthocyanidins content was determined in rice grain of different colors using dimethylamino- cinnamaldehyde （DMACA） staining, Ferric ammonium alum staining and Vanillin staining. Experimental studies showed that the proanthocyanidins mass fraction of five rice breeds （Tannong s/zhi2 l, R288, Fan-14, 12Geng351 and Qingdao2） detected by DMACA staining was successively as follows, 0.136, 0.183, 0.085, 4.115 and 24.736 mg/g （dry weight）; the result detected by Ferric ammonium alum staining was 0.292, 0.094, 1.940 x 104, 1.220 and 8.080 mg/g; and the result detected by Vanillin-HC1 staining was 1.349, 1.363, 1.093, 3.733 and 15.153 mg/g. The proanthocyanidins content of Qingdao2 was the highest, which was about 4.1 to 6.6 times of 12Geng351, and Fan- 14 was the lowest in five rice breeds; the proanthocyanidins content of black rice breeds （Qingdao2 and 12Geng351） was higher than that of white rice breeds （Tannong s/zhi21, R288, Fan-14）. The relative standard deviation （RSD） of the absorbency showed that the precision level of determining proanthocyanidins content as follow, the first was vanillin staining, the second was DMACA staining and the third was ferric ammonium alum staining.