六味地黄汤对IgA肾病大鼠α-SMA及FN表达的影响

Effects of Liuwei Dihuang Decoction onthe Expression of α-SMA and FN in IgA Nephropathy Rats

目的观察六味地黄汤对IgA肾病大鼠α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)及纤维连接蛋白(fibronectin,FN)表达的影响,进一步探讨其可能作用机制。方法 SD大鼠40只随机分为正常对照组、模型组、雷公藤多苷片组,六味地黄汤组。除正常对照组外均采用牛血清白蛋白+脂多糖+四氯化碳方法建立实验性IgA肾病模型。造模成功后分组干预连续灌胃4周,分别在每周末检测尿蛋白定量及尿红细胞计数,第4周末处死各组大鼠,进行肾功能检测。采用免疫组化、Western blot检测各组α-SMA及FN蛋白表达,进行半定量分析。结果各组尿蛋白定量及尿红细胞计数比较,模型组较正常组显著升高(P<0.05),而六味地黄汤组与模型组比较显著降低(P<0.05),差异有统计学意义;各组α-SMA和FN表达,模型组较正常组表达显著升高(P<0.05),而六味地黄汤组较模型组表达下降,(P<0.05)差异有统计学意义。结论六味地黄汤可减少IgA肾病大鼠尿蛋白及尿红细胞数,改善其肾功能,可能与通过下调α-SMA及FN表达相关。

Objective To observe the effects of Liuwei Dihuang Decoction on the expression of α-SMA and fibronectin （FN） in IgA nephropathy rats, and further investigate its possible mechanism. Methods Forty SD rats were equally randomly divided into 4 groups： the normal group, model group, tripterygium glycosides tablets group and Liuwei Dihuang Decoction group. IgAN rat models were established with bovine serum albumin （BSA）, lipopolysaccharide （LPS） and carbon tetrachloride （CC14）. The successfully established models were given 4 weeks of continuous perfusion. The proteinuria and urinary red blood cell count were detected every week, respectively. At fourth weekend, all the rats were killed for kidney function test. The α-SMA and FN protein expression of each group were detected by using immunohistochemistry and Western blot, and semi-quantitative analysis was made. Results The urine protein and urine red blood cell countsin each group were compared, the model group was higher significantly than that of normal group （P 〈0.05）, while compared with mode group, Liuwei Dihuang Decoction group was decreased significantly （P〈0.05）, the difference was statistically significant.The α-SMA and FN expression in model group were increased than that in the normal group, while the expression of Liuwei Dihuang decoction group was decreased comparing with the model group （P〈0.05）, the difference was statistically significant. Conclusion Liuwei Dihuang Decoction can reduce the urine protein and urine red blood cell count of IgA nephropathy rats and improve the kidney function, which may be related with down-regulation of α-SMA and FN expression.