盐酸戊乙奎醚与山莨菪碱对创伤性休克兔脏器损伤的保护作用

Protective effects of penehyclidine hydrochloride and anisodamine against the organ injuries in rabbits with traumatic shock

目的研究盐酸戊乙奎醚（PHC）对创伤性休克兔复苏后重要脏器损伤的保护作用。方法采用Lmnson’s法建立创伤性休克动物模型，24只健康日本长耳大白兔，随机分为对照组（Con组）、生理盐水复苏组（NS组）、PHC处理组（PHC组）、山莨菪碱处理组（ANI组）。动态观察平均动脉压（MAP）和心率（HR），在休克前、休克末和复苏后不同时间点采血测定血乳酸盐（ABL）浓度。实验结束后处死动物取肺脏、肝脏、小肠组织，光镜下观察病理学变化。结果4组动物休克前（T1）MAP比较差异无统计学意义；休克末（T2）NS组、PHC组和ANI组MAP显著降低；复苏后各时间点，PHC组和ANI组MAP均显著回升，NS组MAP未恢复到T1水平，显著低于Con组、PHC组和ANI组。在T1和T2两个时间点，4组动物HR组间比较差异无统计学意义；在复苏后各时间点，Ns组HR显著降低；PHC组HR有所降低，高于Ns组但低于ANI组，差异有统计学意义。T1时点4组动物血浆ABL浓度比较差异无统计学意义；T2和复苏后各时间点，NS组、PHC组、ANI组血浆ABL浓度显著升高，与T1、Con组比较差异均有统计学意义；休克后4个时间点，NS组血浆ABL浓度又显著高于PHC组和ANI组，差异有统计学意义。结论PHC和ANI有助于稳定创伤性休克兔的血流动力学，对创伤性休克兔造成的脏器损伤有确切的保护作用。

Objective To investigate the protective effects of penehyclidine hydrochloride on organ injury in rabbits with traumatic shock. Methods The animal model of traumatic shock was established by Lamson＇s method. Twenty - four rabbits were randomly divided into control group （ Con group）, traumatic shock with normal saline group （NS group ）, traumatic shock treated with penehyclidine hydrochloride group （ PHC group） and anisodamine （ ANI group）. At pre - shock, tele - shock, four timepoints after resuscitation, mean arterial pressure （MAP） and heart rate （HR） were monitored. Arterial blood lactate （ABL） concentration was measured by spectrophotometry. By the end of the experiment, the pathological changes of tissue samples of the liver, lung and intestine were observed under microscope. Results There were no significant differences of MAP at pre - shock among the four groups. At tele - shock, the MAP of all rabbits in NS group, PHC group and ANI group significantly decreased. At eachtime point after resuscitation, the MAP in PHC group and ANI groupsignificantly rose again, but the MAP in NS group did not recover to the level of pre - shock and was significantly lower than that in Con group, PHC group and ANI group. There was no significant group difference in HR at pre - shock and tele - shock. At eachtime point after resuscitation, the HR of rabbits in NS group was significantly decreased. The HR of rabbits in PHC group was slightly decreased, higher than that in NS group but lower than that in ANI group. There were no significant group differences in plasma ABL concentration at pro - shock time point. Compared with pre - shock and control group, the plasma ABL concentration in NS group, PHC group and ANI groupwas significantly increased; at tele - shock and each time point after resuscitation. The plasma ABL concentration was significantly higher in NS group than that in PHC and ANI group. The results of pathology showed that tissue injury of rabbits in PHC and ANI groups was significantly reduced compared with NS group. Conclusion PHC and ANI can stabilize hemodynamics in rabbits with traumatic shock. There is a protective effect on organ injury induced by traumatic shock.